Objective To analyze the testing data of settling microbe detection in the pharmacy intravenous admixture service(PIVAS)center of a hospital and to evaluate the cleanliness of clean areas.To ensure the environmental indicators of clean areas and to facilitate a fine management.Methods The detection results of settling microbe in different clean areas of a PIVAS center from 2019 to 2021 were analyzed and compared.Results The frequency of the three-year pass rate of the operating station was mostly concentrated in 90%and above,and the three-year pass rate of handling room all exceeded 90%.The t-test of pairwise comparison of the pass rate of operating stations showed a significant annual increase(P<0.05).There was no significant correlation between the detection results of the operating station and the testing month(P<0.05).Conclusion The factors affecting the detection results of settling microbe are complicated.Strict implementations of the required specifications ensure the accuracy of the testing results and evaluation of the cleanliness of the clean areas.