Effects of ropivacaine on the proliferation,apoptosis and migration of esophageal cancer cells by inhibiting the Ras/Raf/MEK/ERK signaling pathway
Objective To explore the effects of ropivacaine on the proliferation,apoptosis and migration of esophageal cancer cells KYSE-150,and the underlying mechanism.Methods KYSE-150 cells were cultured in vitro.They were treated with blank control,low-dose ropivacaine(10μg/mL),medium-dose ropivacaine(20μg/mL)and high-dose ropivacaine(50μg/mL).Cell proliferation,apoptosis and migration were detected by CCK-8 assay,flow cytometry and wound healing assay,respectively.Western blotting was used to detect the protein levels of Ras,Raf,MEK,ERK,cell proliferation-related nuclear antigen(Ki-67),Bcl-2 associated X protein(Bax),and B-cell lymphoma genes-2(Bcl-2),matrix metalloproteinase 2(MMP-2),and matrix metalloproteinase 9(MMP-9).Results Compared with those of blank control,KYSE-150 cells induced with low-dose,medium-dose and high-dose ropivacaine presented significantly lower cell survival,wound healing rate and protein expressions of Ki-67,Bcl-2,MMP-2,MMP-9,Ras,Raf,MEK and ERK,but significantly higher apoptotic rate and protein level of Bax in a dose-dependent manner(P＜0.05).Conclusion Ropivacaine can inhibit the proliferation and migration of KYSE-150 cells and promote their apoptosis by inhibiting the Ras/Raf/MEK/ERK signaling pathway.
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