Effects of lncRNA PVT1 interference on the proliferation and apoptosis of acute myeloid leukemia cells
Objective To investigate the effect of long non-coding RNA plasmacytoma variant translocation 1(lncRNA PVT1)on the proliferation and apoptosis of the line human leukemia cell line(HL-60).Methods A total of 106 patients with acute myeloid leukemia(AML)were selected as the experimental group,and 100 healthy volunteers during the same period were selected as the control group.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was used to detect the plasma expression of lncRNA PVT1.HL-60 cells were treated with blank control,or transfected with si-lncRNA PVT1 or si-Control.LncRNA PVT1 level was detected by RT-qPCR.Cell proliferative rate was measured by CCK-8 assay.Cell cycle progression and apoptosis were examined by flow cytometry.Expression levels of cell cycle related protein D1(Cyclin D1),cyclin-dependent kinase inhibitor 1 A(P21),B-cell lymphoma-2(BCL-2),Bcl-2 related X protein(Bax),and aspartate proteolytic enzyme 3(Caspase 3)were detected by Western blot and RT-qPCR.Results Plasma lncRNA PVT1 was significantly higher in the experimental group than that of control group(P＜0.05).Knockdown of lncRNA PVT1 significantly downregulated its level in HL-60 cells than those transfected with si-Control(P＜0.05).Knockdown of lncRNA PVT1 significantly decreased cell proliferative rate,arrested cell cycle in G1 phase and induced apoptosis in HL-60 cells(P＜0.05).Compared with those transfected with si-Control,transfection of si-lncRNA PVT1 significantly downregulated Cyclin D1 and Bcl-2,but upregulated P21,Bax and Caspase 3(P＜0.05).Conclusion Knockdown of lncRNA PVT1 can inhibit the proliferation and induce apoptosis of HL-60 cells,suggesting that lncRNA PVT1 may serve as a molecular target for the prevention and treatment of AML.
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