Expression levels of miR-34a-5p and HMGB1 in cervical cancers and precancerous lesions of cervical tissue and their correlation with HPV infection
Objective To investigate the expression levels of microRNA-34a-5p(miR-34a-5p)and high mobility group box protein B1(HMGB1)in cervical cancer(CC)and precancerous lesions and their relationship with human papilloma virus(HPV)infection.Methods A total of 165 cervical surgical specimens in our hospital from January 2018 to June 2022 were retrospectively analyzed,including 55 CC specimens,55 precancerous lesions and 55 normal cervical tissues.The expression levels of miR-34a-5p and HMGB1 in clinical specimens,and HPV infection in all subjects were detected.The correlation of the expression levels of miR-34a-5p and HMGB1 in cervical tissue with HPV infection in patients with CC and cervical precancerous lesions was analyzed.The relationship between miR-34a-5p and HMGB1 was predicted by bioinformatics,and the correlation of expression level of miR-34a-5p with that of HMGB1 in cervical tissue of patients with CC and cervical precancerous lesions was analyzed.Results The expression level of miR-34a-5p in CC tissues was significantly lower than that of normal cervical tissues and precancerous lesions(P＜0.05),while the expression level of HMGB1 and the positive infection rate of HPV were higher(P＜0.05).The expression level of miR-34a-5p in cervical precancerous lesions was significantly lower than that of normal cervical tissues(P＜0.05),while the expression level of HMGB1 and the positive infection rate of HPV were significantly higher(P＜0.05).The expression level of miR-34a-5p in cervical tissue was negatively correlated with HPV infection in patients with CC and precancerous lesions(P＜0.05).The expression level of HMGB1 in cervical tissue was positively correlated with HPV infection in patients with CC and precancerous lesions(P＜0.05).MiR-34a-5p had a binding site with HMGB1,and the expression level of miR-34a-5p in precancerous lesions of CC and CC specimens was negatively correlated with that of HMGB1(P＜0.05).Conclusion MiR-34a-5p is down-regulated and HMGB1 is up-regulated in CC and precancerous lesions,both of which are closely related to HPV infection.
microRNA-34a-5pcervical cancerhigh mobility group protein box 1precancerous lesionshuman papilloma virus
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NSTL
万方数据
