Objective To investigate the clinical value of high-throughput sequencing in an early diagnosis of cervical cancers.Methods A total of 45 cervical cancer patients admitted to hospital were selected as the research objects.Cervical exfoliated cells were collected for DNA extraction and high-through sequencing.Gene mutations in cervical exfoliated cells of cervical cancer patients were detected,and relevant mutation data were searched in The Cancer Genome Atlas(TCGA)and Catalogue Of Somatic Mutations In Cancer(COSMIC)databases.Results Totally 42 out of 45(93.33％)cervical cancer patients carried gene mutations.A total of 160 mutant genes were identified in cervical cancer patients,with the majority of PIK3CA gene mutations,followed by Rb1,AKT2 and FAT1 gene mutations.Classified by the type of gene mutations,we detected copy number mutation,insertion deletion mutation,frameshift mutation,nonsense mutation and missense mutation in cervical cancer patients,with copy number mutation and missense mutation dominantly.Meanwhile,PIK3CA gene mutation was significantly correlated with the differentiation degree of cervical cancer(P＜0.05),but not correlated with high-risk HPV infection,lymph node metastasis,histological type,clinical stage,tumor size and age(P＞0.05).Conclusion Gene mutations,especially polygenic mutations,are common in cervical cancer patients.PIK3CA is a common mutant gene detected in cervical cancer,with the major involvement of the PI3K-Akt signaling pathway.The use of high-throughput sequencing is able to identify novel gene mutations,providing a theoretical foundation for an early diagnosis of cervical cancers.
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