Alpinia Oxyphylla Fructus prevents and treats diabetic nephropathy via the RhoA/ROCK signaling pathway
Objective To study the prevention and treatment effect of Alpinia Oxyphylla Fructus(AOF)on diabetic nephropathy and the underlying mechanism.Methods HK-2 cells were induced with high-glucose medium(30mmol/L)for 48 h,and induced with canagliflozin or AOF for 24 h.Cells induced with normal medium were taken as negative controls.The protein and mRNA levels of key genes in the ras homolog gene family member A(RhoA)/Rho-associated kinase(ROCK)signaling pathway were detected by Western blot and quantitative polymerase chain reaction(qPCR),respectively.Cell apoptosis,proliferation and oxidative stress were detected by flow cytometry,cell counting kit-8(CCK-8)assay and reactive oxygen species(ROS)measurement kit,respectively.Results Compared with those induced with normal medium,high-glucose-induced HK-2 cells showed significantly lower viability,and higher apoptotic rate,ROS level and protein and mRNA levels of key genes in the RhoA/ROCK signaling pathway(P＜0.05).AOF-induced HK-2 cells in high-glucose medium had significantly higher viability,and lower apoptotic rate,ROS level and protein and mRNA levels of key genes in the RhoA/ROCK signaling pathway(P＜0.05).Conclusion AOF promotes cell proliferation and inhibits apoptosis of HK-2 cells by inactivating the RhoA/ROCK signaling pathway.AOF exerts a therapeutic efficacy on diabetic nephropathy by reducing inflammatory response,oxidative stress and fibrosis of renal tubular epithelial cells.
Alpinia Oxyphylla Fructusdiabetic nephropathyras homolog gene family member A(RhoA)/Rho-associated kinase(ROCK)
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