Inhibitory effect of oleuropein on proliferation and apoptosis of human osteosarcoma MG-63 cells
Objective To study the effect of oleuropein on the activity of human osteosarcoma MG-63 cells.Methods MG-63 cells were dissolved in Polybutylene Succinate(PBS)and prepared with different concentrations of oleuropein(0,25,50,100,200,400μg/ml)to treat MG-63 cells for 24h or 48h,and the changes in cell activity and proliferation ability were analyzed by cell counting Kit-8(CCK8)and clone formation assays.Annexin-V/PI double staining method was performed to analyze the cells apoptosis level.Results After treatment with oleuropein,the activity and proliferation of MG-63 cells were significantly reduced,and apoptosis level was significantly increased(P＜0.05).The 48-h inhibitory concentration 50％(IC50)was significantly lower than 24-h IC50.The inhibitory effect on tumor cells was positively correlated with the concentration and treatment time of oleuropein(P＜0.05).Conclusion Oleuropein can significantly inhibit the activity and proliferation of human osteosarcoma cell line MG-63,which can induce MG-63 apoptosis.
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