Effects of sevoflurane on lung function and the NF-κB/NLRP3 inflammatory pathway in rats with acute lung injury
Objective To study the effects of sevoflurane on lung function and the nuclear factor-KB/nucleotide binding oligomerization domain like receptor protein 3(NF-κB/NLRP3)pathway in rats with acute lung injury.Methods A total of 75 rats were randomly divided into control group,model group,and 1.5％,2.0％,and 2.5％sevoflurane groups,with 15 rats in each group.Except for those in the control group,acute lung injury models were prepared by intraperitoneal injection of 5mg/kg lipopolysaccharide into the other groups.After modeling,rats in the 1.5％,2.0％,and 2.5％sevoflurane groups freely inhaled 1.5％,2.0％,and 2.5％sevoflurane for 60 minutes,respectively,while those in the control group and model group freely breathed air.General situation,lung function status(airway resistance,dynamic lung compliance,forced lung capacity),lung wet-to-dry ratio(W/D),oxygenation index(arterial partial pressure of oxygen[PaO2]/inhalation oxygen concentration[FiO2]),pathological changes in lung tissues,and levels of inflammatory factors in alveolar lavage fluid(tumor necrosis factor[TNF-α],interleukin-6[IL-6],interleukin-1β[IL-1β])were compared.Protein levels of NF-κB,NLRP3,ASC(apoptosis-associated speck-like protein containing a caspase recruitment domain),and caspase-1 in lung tissue of rats in each group were detected by Western blot.Results After 96 hours of lipopolysaccharide treatment,the survival rate of rats in the control group,model group,and 1.5％,2.0％,and 2.5％sevoflurane groups was 100％(15/15),60.00％(9/15),66.67％(10/15),80.00％(12/15),and 73.33％(11/15),respectively.Induction of 2.0％sevoflurane significantly improved the survival rate of rats.H&E staining showed pronounced pathological changes in the lung tissue of the model group,including a large number of inflammatory cell infiltration,thickening of alveolar walls,and obstruction of alveolar blood vessels.Compared with those of the model group,pathological changes in the lung tissue of rats in the 1.5％,2.0％,and 2.5％sevoflurane groups were alleviated to varying degrees,especially in the 2.0％sevoflurane group.Compared with those of the control group,the model group showed significantly increased airway resistance,lung W/D,and expression levels of TNF-α,IL-1β,IL-6,NF-κB,NLRP3,ASC,and caspase-1(P＜0.05),while dynamic lung compliance,forced lung capacity,and PaO2/FiO2 significantly decreased(P＜0.05).Compared with those of the model group,the 1.5％,2.0％,and 2.5％sevoflurane groups showed significantly lower airway resistance,lung W/D and expression levels of TNF-α,IL-1 β,IL-6,NF-κB,NLRP3,ASC,and caspase-1(P＜0.05),while dynamic lung compliance,forced lung capacity,and PaO2/FiO2 were significantly higher(P＜0.05).Conclusion Heptaflurane has a protective effect on lung function in rats with acute lung injury.The induction of 2.0％heptaflurane has the best lung protection effect by inhibiting the NF-κB/NLRP3 inflammatory pathway.
sevofluraneacute lung injuryratslung functioninflammatory factorslung tissue pathologynuclear factor-κB/nucleotide binding oligomerization domain like receptor protein 3(NF-κB/NLRP3)pathway
万方数据
维普
