首页|乙型肝炎病毒X基因通过抑制Caspase-1表达促进肝癌细胞增殖

乙型肝炎病毒X基因通过抑制Caspase-1表达促进肝癌细胞增殖

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目的 探究乙型肝炎病毒X基因(HBx)对HepG2细胞中Caspase-1介导的肝细胞增殖的影响.方法 用脂质体转染法将HBx真核表达载体pCDNA3.1-HBx瞬时转入HepG2细胞,以阴性载体转染的HepG2细胞(NC)为对照.转染后72 h收集细胞,通过RT-PCR检测HBx的表达,通过qPCR和Western blot法检测Caspase-1的mRNA和蛋白质表达,并通过CCK-8检测HepG2-HBx和HepG2-NC的增殖情况.结果 RT-PCR证实HBx成功转染HepG2细胞株,HepG2-HBx细胞中Caspase-1的mRNA和蛋白表达水平显著低于HepG2-NC组.HepG2-HBx细胞的增殖能力显著高于HepG2-NC组.结论 HBx通过抑制Caspase-1的表达诱导HepG2细胞增殖.
Hepatitis B virus X gene promotes the proliferation of hepatic cells by downregulating Caspase-1
Objective To investigate the effect of hepatitis B virus X gene(HBx)on the proliferation of HepG2 cells mediated by Caspase-1.Methods The pcDNA 3.1 vector loading Hbx(pcDNA3.1-HBx)was transfected into HepG2 cells using lipofectamine,with those transfected with negative vector as negative control(NC).Cells were collected 72h after transfection.The expression of HBx was detected by reverse transcription-polymerase chain reaction(RT-PCR).The mRNA and protein levels of Caspase-1 were detected by quantitative polymerase chain reaction(qPCR)and Western blot,respectively.The proliferation of HepG2 cells transfected with pcDNA3.1-HBx and NC was assessed by CCK-8 assay.Results Transfection of pcDNA3.1-HBx was success with the verification by RT-PCR.The mRNA and protein levels of Caspase-1 were significantly lower in HepG2 cells transfected with pcDNA3.1-HBx than those transfected with NC.The proliferative rate was significantly higher in HepG2 cells transfected with pcDNA3.1-HBx than those transfected with NC.Conclusion HBx promotes the proliferation of HepG2 cells by downregulating Caspase-1.

hepatitis B virus X(HBx)geneHepG2 cellsCaspase-1cell proliferation

韦武均、王春芳、钟丽梅、黄晶晶、唐霏林、黄艳、黄英新、陆艳珍

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533000 广西壮族自治区百色市,右江民族医学院附属医院检验科

533000 广西壮族自治区百色市,右江民族医学院附属医院急诊科

广西壮族自治区百色市妇幼保健院保健科

HBx基因 HepG2细胞 Caspase-1 细胞增殖

国家自然科学基金项目广西卫生健康委员会自筹经费科研课题广西卫生健康委员会自筹经费科研课题百色市科学研究与技术开发计划项目广西高校中青年教师科研基础能力提升项目中医药自筹经费科研课题广西肝胆疾病分子病理学重点实验室开放课题

81960303Z20211114Z20190202百科202132422021KY0538GXZYL20220304GXZDSYS-009

2024

10.3969/j.issn.1002-7386.2024.19.009

河北医药
河北省医学情报研究所

河北医药

CSTPCD
影响因子：1.075
ISSN：1002-7386
年,卷(期)：2024.46(19)