Research on the mechanism of cancer-associated fibroblast extracellular vesicles carrying miRNA-95 to promote the invasion and migration of ovarian cancer cells
Objective To explore the effect of cancer-associated fibroblasts(CAFs)-derived exosomal miRNA-95 on the migration and invasion of ovarian cancer cells.Methods Normal fibroblasts(NFs)and CAFs were collected from tumor and cancer-adjacent tissues of ovarian cancer patients.These cell-derived exosomes were characterized by Western blot and transmission electron microscope(TEM).The miRNA-95 expression was assessed by quantitative reverse-transcription polymerase chain reaction(qRT-PCR).Transwell was used to evaluate cell migration and invasion,and Hoechst staining was used to monitor the apoptosis rate of tumor cells.Results Both CAFs and NFs exosomes can enter ovarian cancer cells,and CAFs-derived exosomes promoted the migration and invasion of ovarian cancer cells more than NFs-derived exosomes.The miRNA-95 was upregulated in CAFs-derived exosomes,and CAFs-derived exosome miRNA-95 promoted ovarian cancer cell migration and invasion,and induced epithelial-mesenchymal transition(EMT).Conclusion Our results demonstrated that CAFs-derived exosomal miRNA-95 can promote the migration,invasion and metastasis of ovarian cancer cells.Targeting CAFs-derived exosomal miRNA-95 may serve as a potential option to promote therapeutic progress in ovarian cancer.
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维普
