Protective effects of spironolactone on high-glucose and aldosterone-induced injury of human renal podocyte by inhibiting VEGF
Objective This study aims to investigate the renoprotective mechanism of spironolactone(SPI)by studying its effects on the proliferation,apoptosis,and vascular endothelial growth factor(VEGF)expression of podocytes induced by high glucose and aldosterone(ALD).Methods Human podocytes were treated with high glucose and ALD at varying concentrations,and subsequently intervened with SPI.The proliferation and apoptosis of podocytes were assessed using CCK-8 and flow cytometry,respectively.Expression level of VEGF was detected by quantitative real-time polymerase chain reaction(qPCR)and Western blot.Results Compared with those treated with normal glucose concentration,high-glucose treatment significantly inhibited proliferation and increased apoptosis of podocytes(P＜0.05).Induction of ALD at varying concentrations inhibited the proliferation of podocytes in a dose-dependent manner(P＜0.05).Induction of high glucose and ALD significantly upregulated mRNA and protein expression of VEGF in podocytes(P＜0.05).SPI intervention significantly attenuated the effects of ALD on podocyte proliferation and apoptosis,and reversed the promotional effect of high-glucose and ALD treatment on VEGF expression(P＜0.05).Conclusion SPI may exert renoprotective effects by inhibiting the expression of VEGF,thereby reversing the inhibitory effect of high-glucose and ALD on inhibiting proliferation and promoting apoptosis of podocytes.
NETL
NSTL
万方数据
