The Study on Influencing Factors of in Vitro Culture System of Cucumber Unpollinated Ovary
Taking cucumber species,'Fei Cui''Jin You 35''Rui Guang 2'and'Jing Feng 268'as test materials,influencing factors of in vitro culture of cucumber unpollinated ovary were studied to screen out the embryo induction system suitable for various genotypes of cucumber.The results showed that in different com-binations of the four genotypes,the embryo incidence of unpollinated ovary of cucumber was the highest in 35℃ heat shock treatment for 2 days with 0.07 mg·L-1 TDZ in MS medium 2 days before flowering,which was 60.8％,20.8％,43.3％and 65.0％,respectively.There was a significant difference in the incidence of embryos among different genotypes,the incidence of'Jing Feng 268'was the highest with 18.04％,and that of'Rui Guang 2'was the lowest,with 5.23％.The effect of heat shock treatment at 35℃for 2 days was the best,and the incidence of unpollinated ovary embryo was the highest 2 days before flowering.The embryo in-cidence rate of'Fei Cui'was the highest when treated with 0.09 mg·L-1 TDZ,and that of'Rui Guang 2''Jin You 35'and'Jing Feng 268'was the highest when treated with 0.07 mg·L-1 TDZ.
万方数据
维普
