Effects of Pro-longed Water Stress on Physiological and Biochemical Characteristics,Antioxidant Enzymes and Expression of Genes related to Biosynthesis of Active Ingredients of Atractylodes chinensis(DC.)Koidz Seedlings
The physiological,biochemical,and molecular characteristics of Atractylodes chinensis(DC.)Koidz seedlings responding to pro-longed water stress were studied with normal water management as a control and a single-factor-fully-randomized experimental design under conditions of 18-day submersion and 10-day normal water management to reveal the physiological and biochemical characteristics,root activity and anatom-ical structure,antioxidant enzymes activity and their relative expression,and a key enzyme ACC in the biosyn-thesis of active ingredients of A.chinensis seedlings.The results showed that after continuous submersion for 2～18 days,the relative water content of A.chinensis seedlings was significantly higher(P＜0.05)than that of the control on the 2nd,4th,10th,16th,and 18th days of submersion.The soluble protein mass concentra-tion,proline mass fraction,and molar mass concentration of malondialdehyde significantly increased and then decreased with the increase of submerging time,which was significantly different from the control.The aerated tissue was bigger as submerging time increased.The activity of SOD,POD,and CAT significantly increased and then decreased,the relative expression characteristics of the genes differed significantly from that of the control group,the relative expression levels of SOD and CAT reached their highest values on the 4th day of submersion treatment,while the relative expression levels of POD reached their highest values on the 10th day of submersion treatment.The relative expression level of ACC,a key enzyme in the biosynthesis of active in-gredients,was significantly different from the control,and reached its highest value on the 14th day of submer-sion.After 10 days of recovery,physiological and biochemical indicators,antioxidant enzyme activity,and relative expression levels of related genes were all restored,but there were still significant or extremely signifi-cant difference from the control group in the mass concentration of soluble protein,mass fraction of proline,and molar mass concentration of malondialdehyde.The ventilation tissue was significantly reduced.The A.chinensis seedlings were adapted to pro-longed water stress by regulating osmotic substances,antioxidant en-zyme activity,related gene expression,and changes in root tissue structure.
Atractylodes chinensis(DC.)Koidzpro-longed water stressphysiological and biochemical char-acteristicsantioxidant enzymeqRT-PCR
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