河北科技师范学院学报2024，Vol.38Issue(3) ：37-42.DOI:10.3969/J.ISSN.1672-7983.2024.03.006

锦鲤体表溃烂病病原菌分离鉴定及中药敏感性测定

Pathogen Isolation and Identification of Cyprinus carpio haematopterus Skin Ulcer Disease and Sensitivity Test of Chinese Herbal Medicines

任海 李翠兰 张志业 王娜 高桂生 王颖婕
河北科技师范学院学报2024，Vol.38Issue(3) ：37-42.DOI:10.3969/J.ISSN.1672-7983.2024.03.006
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  • 万方数据

锦鲤体表溃烂病病原菌分离鉴定及中药敏感性测定

Pathogen Isolation and Identification of Cyprinus carpio haematopterus Skin Ulcer Disease and Sensitivity Test of Chinese Herbal Medicines

任海 1李翠兰 2张志业 2王娜 2高桂生 1王颖婕2
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作者信息

  • 1. 河北科技师范学院海洋资源与环境学院,河北 秦皇岛,066600;河北省海洋动力过程与资源环境重点实验室,河北秦皇岛,066004
  • 2. 河北科技师范学院海洋资源与环境学院,河北 秦皇岛,066600
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摘要

为明确引起锦鲤(Cyprinus carpio haematopterus)体表溃烂病的病原菌,并获得有效单味中草药.本次试验从患病锦鲤体表病灶溃烂处分离获得1株优势菌,通过革兰氏染色、理化鉴定、16SrDNA序列分析、致病性检测和中药敏感性试验.结果显示,该优势菌为腐败希瓦氏菌(Shewanella putrefaciens),革兰氏阴性菌,命名为CC-1.动物回接感染试验证实,该菌为锦鲤的致病菌.中草药药敏试验结果显示,该菌对五倍子、苏木、黄连等高敏感性,对黄芪、知母、金荞麦等中度敏感,对茯苓和虎杖不敏感;选择上述具有抑菌作用的13味中草药水煎剂检测最小抑菌浓度(MIC)和最小杀菌浓度(MBC),结果显示,苏木、黄芩、乌梅、五倍子的MIC和MBC分别为7.81 mg/mL、15.63 mg/mL;黄连次之,其MIC和MBC分别为15.63 mg/mL、31.25 mg/mL.

Abstract

The pathogenic bacteria causing skin ulcer of Cyprinus carpio haematopterus were determined and effective Chinese herbal medicines were obtained.The animal infection test confirmed that Shewanella putrefaciens,a Gram-negative bacterium named CC-1 that was isolated from the ulcer tissue of the diseased K.carp was the pathogenic bacterium through gram staining,physiological and biochemical identification,16Sr DNA sequence analysis,pathogenicity detection,and Chinese herbal medicine sensitivity test.The Chinese herbal medicine sensitivity test showed that S.putrefaciens was highly sensitive to Galla chinensis,Sappan lignum,and Coptidis rhizome,moderately sensitive to Astragalus membranaceus,Anemarrhena asphodeloides Bunge,and Rhizoma fagopyri cymosi,and insensitive to Poria cocos and Polygonum cuspidatum.The MIC(minimum inhibitory concentration)and MBC(minimum bactericidal concentration)of 13 Chinese herbs with antibacterial effects were determined.The results showed that the MIC and MBC of S.lignum,Scutellariae radix,Fructus mume,and G.chinensis were 7.81 mg/mL and 15.63 mg/mL respectively,followed by C.rhizoma with the MIC and MBC of 15.63 mg/mL and 31.25 mg/mL respectively.

关键词

锦鲤/体表溃烂病/腐败希瓦氏菌/分离鉴定/中药敏感性

Key words

Cyprinus carpio haematopterus/skin ulcer disease/Shewanella putrefaciens/isolation and identification/sensitivity of Chinese herbal medicine

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出版年

2024
河北科技师范学院学报
河北科技师范学院

河北科技师范学院学报

影响因子：0.414
ISSN：1672-7983
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