Objective:To explore the protective effect of Tongping Zhigan Formula on high-fat-diet-induced non-alcoholic fatty liv-er disease(NAFLD)in model mice and its related mechanisms of inhibiting inflammation,based on myeloid differentiation factor 88(MyD88)/nuclear factor-κ B(NF-κ B)pathway mediated by Toll-like receptor 4(TLR4).Methods:C57BL/6J male mice were ran-domly divided into normal group,model group,pioglitazone hydrochloride group,and low-,medium-,and high-dose groups of Tong-ping Zhigan Formula,with 10 mice in each group.Samples were collected after 8 weeks of administration.Results:Compared with the normal group,the indicators in model group,including body mass,blood lipids,liver function,IL-6,TNF-α,and TLR4,MyD88,Ikk β,NF-κ B protein,and mRNA expressions of TLR4,MyD88 and Ikk β,increased significantly(P＜0.05);The above indicators of the midium-and high-dose groups of Tongping Zhigan Formula significantly improved(P＜0.05).A large amount of fat accumulated in the liver cells in the model group,with ballooning degeneration;The cytoplasm was compressed by spherical lipid droplets and infil-trated by inflammatory cells;The pathological morphology of liver tissue in each medication group was improved,and the infiltration of spherical lipid droplets and inflammatory cells were reduced.Conclusion:Tongping Zhigan Formula can regulate blood lipid levels in NAFLD mice induced by high-fat diet,improve liver function,reduce liver lipid accumulation,alleviate fatty liver and liver damage,and alleviate inflammation.Its anti-inflammatory mechanism may be related to its activating TLR4-mediated MyD88/NF-κ B signaling pathway,inhibiting lipid synthesis and the expression of inflammatory factors.
Non-alcoholic fatty liver diseaseinflammationTongping Zhigan FormulaTLR4MyD88IkkβNF-κ B
NETL
NSTL
维普
万方数据
