Objective:To explore the effect of Huazhuo Jiedu Granules(granules for eliminating turbid and removing toxin)on in-flammation related indicators in rats with acute lung injury(ALI).Methods:Sixty rats were divided into blank group,model group,prednisone group,low-,medium-,and high-dose groups of Huazhuo Jiedu Granules.After 7 days of administration,ALI model rats were constructed.Observation of lung tissue pathology and lung injury score were used to measure lung tissue wet/dry weight ratio(W/D),lung permeability index,arterial partial pressure of oxygen(PaO2),classification count of inflammatory cells in bronchoalveolar lavage fluid(BALF),lung tissue nuclear factor κ B(NF-κ B)P65,Tumor Necrosis Factor α(TNF-α),mRNA expression of inter-leukin-6(IL-6)and interleukin-10(IL-10),and to detect interleukin-1 β(IL-1β)in lung tissue and macrophage inflammatory pro-tein-1a(MIP-1a).Results:Compared with the blank group,the lung tissue structure of the model group was disordered,with intersti-tial edema and inflammatory cell infiltration;At the same time,lung injury score,lung tissue W/D,lung permeability index,BALF inflammatory cell count increased(P＜0.05),PaO2 decreased(P＜0.05),and NF-κ B p65,TNF-α,IL-6 mRNA expression in-creased(P＜0.05),IL-10 mRNA expression decreased(P＜0.05),IL-1 β and MIP-1a increased(P＜0.05).Compared with the model group,the low-,medium-,and high-dose groups of Huazhuo Jiedu Granules showed that inflammatory cell infiltration and edema in lung tissue in varying degrees,and lung injury score,lung tissue W/D,lung permeability index,and BALF inflammatory cell count decreased(P＜0.05),PaO2(P＜0.05)increased,and mRNA expression of NF-κ B p65 in lung tissue reduced(P＜0.05),IL-1 β and MIP-1a decreased(P＜0.05);mRNA expression of IL-6 and TNF-α Medium-and high-dose groups decreased(P＜0.05),while IL-10 mRNA expression increased(P＜0.05).Conclusion:Huazhuo Jiedu Granules can improve the pathological damage of lung tissue in ALI model rats,and its mechanism may be related to regulating the levels of inflammatory factors in the lungs.
NETL
NSTL
维普
万方数据
