摘要
目的:探讨化浊解毒颗粒对急性肺损伤(Acute lung injury,ALI)大鼠炎症相关指标的影响.方法:60 只大鼠随机分为空白组,模型组,泼尼松组,化浊解毒低、中、高剂量组.除空白组外,其余各组给药 7d后构建ALI模型.行肺组织病理学观察及肺损伤评分,测定肺组织湿/干重比(W/D)、肺通透性指数、动脉血氧分压(PaO2)、肺泡灌洗液(BALF)炎性细胞分类计数,肺组织核因子κB(NF-κB)p65、肿瘤坏死因子α(TNF-α)、白细胞介素 6(IL-6)、白细胞介素 10(IL-10)mRNA表达,并检测肺组织白细胞介素 1β(IL-1β)、巨噬细胞炎性蛋白 1a(MIP-1a)含量.结果:与空白组比较,模型组大鼠肺组织结构紊乱,间质水肿,炎性细胞浸润;同时肺损伤评分、肺组织W/D、肺通透性指数、BALF炎性细胞数目,肺组织NF-κB p65、TNF-α、IL-6 mRNA表达,IL-1β、MIP-1a水平均升高(P＜0.05),PaO2、IL-10 mRNA表达均降低(P＜0.05).与模型组相比,化浊解毒低、中、高剂量组肺组织炎性细胞浸润及水肿不同程度减轻,且肺损伤评分、肺组织W/D、肺通透性指数、BALF炎性细胞数目、肺组织NF-κB p65 mRNA表达、IL-1β、MIP-1a水平均降低(P＜0.05),PaO2升高(P＜0.05);化浊解毒中、高剂量组肺组织IL-6 和TNF-α mRNA表达降低(P＜0.05),IL-10 mRNA表达升高(P＜0.05).结论:化浊解毒颗粒能改善ALI模型大鼠肺组织病理损伤,其机制可能与调控肺内炎症因子水平有关.
Abstract
Objective:To explore the effect of Huazhuo Jiedu Granules(granules for eliminating turbid and removing toxin)on in-flammation related indicators in rats with acute lung injury(ALI).Methods:Sixty rats were divided into blank group,model group,prednisone group,low-,medium-,and high-dose groups of Huazhuo Jiedu Granules.After 7 days of administration,ALI model rats were constructed.Observation of lung tissue pathology and lung injury score were used to measure lung tissue wet/dry weight ratio(W/D),lung permeability index,arterial partial pressure of oxygen(PaO2),classification count of inflammatory cells in bronchoalveolar lavage fluid(BALF),lung tissue nuclear factor κ B(NF-κ B)P65,Tumor Necrosis Factor α(TNF-α),mRNA expression of inter-leukin-6(IL-6)and interleukin-10(IL-10),and to detect interleukin-1 β(IL-1β)in lung tissue and macrophage inflammatory pro-tein-1a(MIP-1a).Results:Compared with the blank group,the lung tissue structure of the model group was disordered,with intersti-tial edema and inflammatory cell infiltration;At the same time,lung injury score,lung tissue W/D,lung permeability index,BALF inflammatory cell count increased(P＜0.05),PaO2 decreased(P＜0.05),and NF-κ B p65,TNF-α,IL-6 mRNA expression in-creased(P＜0.05),IL-10 mRNA expression decreased(P＜0.05),IL-1 β and MIP-1a increased(P＜0.05).Compared with the model group,the low-,medium-,and high-dose groups of Huazhuo Jiedu Granules showed that inflammatory cell infiltration and edema in lung tissue in varying degrees,and lung injury score,lung tissue W/D,lung permeability index,and BALF inflammatory cell count decreased(P＜0.05),PaO2(P＜0.05)increased,and mRNA expression of NF-κ B p65 in lung tissue reduced(P＜0.05),IL-1 β and MIP-1a decreased(P＜0.05);mRNA expression of IL-6 and TNF-α Medium-and high-dose groups decreased(P＜0.05),while IL-10 mRNA expression increased(P＜0.05).Conclusion:Huazhuo Jiedu Granules can improve the pathological damage of lung tissue in ALI model rats,and its mechanism may be related to regulating the levels of inflammatory factors in the lungs.
国家科技期刊平台
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维普
万方数据