Objective:To investigate the mechanism of the effective component of Chinese medicine Fangji(tetrandrine)in im-proving oxidative stress response in early brain injury(EBI)after subarachnoid hemorrhage(SAH)in rats.Methods:Thirty-six male SD rats were randomly divided into sham operation group(Sham group),model group(Model group),and tetrandrine group(Tet group).The SAH model was established using intracranial vascular puncture,and the Tet group received intraperitoneal injection of tetrandrine(20 mg/kg)after surgery.The SAH bleeding score,neurological deficit score,and brain water content were measured in each group.Nissl staining and HE staining were used to observe the morphology and quantity of neurons in the cerebral cor-tex.Transmission electron microscopy was used to observe the structural changes of mitochondria in the brain tissue.Biochemical meth-ods were employed to detect the levels of reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px).Results:Compared with the Sham group,the Model group exhibited aggravated brain tissue damage under microscopic observation,increased brain water content,ROS,and MDA levels,and decreased neurological deficit scores,SOD,and GSH-Px levels(P＜0.05).In contrast to the Model group,the Tet group showed reduced brain tissue damage un-der microscopic observation,decreased brain water content,ROS,and MDA levels,and increased neurological deficit scores,SOD,and GSH-Px levels(P＜0.05).Conclusion:The effective component of Chinese medicine Fangjican inhibit oxidative stress response and exert protective effects on early brain injury after SAH in rats.
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