Objective:To compare the therapeutic effects of Buyang Huanwu Decoction and Astragaloside IV in the treatment of ischemic stroke in rats.Methods:Fifty SD rats were randomly divided into five groups:sham operation group(Sham group),middle cerebral artery occlusion model group(MCAO group),Buyang Huanwu Decoction group(BYHWD group),Astragaloside IV group(AS-IV group),and Xue Sai Tong group,with 10 rats in each group.The middle cerebral artery occlusion(MCAO)model was es-tablished in rats.The Zea Longa method was used to assess the degree of neurological cleficit,TTC staining to detect the volume of cer-ebral infarction;HE staining to observe pathological morphological changes in nerve cells;Nissl staining to observe changes in Nissl bodies;Immunohistochemical staining and immunofluorescence staining to observe and compare the expression of neuron-specific nucle-ar protein NeuN.Results:Compared with the MCAO group,both the BYHWD group and the AS-IV group showed improvements in neurological deficit symptoms(P＜0.05),reduced cerebral infarction volume(P＜0.05),regular neuronal cell morphology,increased Nissl body counts(P＜0.05),andelevated NeuNproteinexpression(P＜0.05).When compared with the AS-IV group,the BYHWD group displayed a slight reduction in neurological deficit symptoms,but the difference was not statistically significant(P＞0.05).However,the BYHWD group showed more significant therapeutic effects in other detected parameters(P＜0.05).Conclusion:Both BYHWD and Astragaloside IV can alleviate the neurological deficit in MCAO rats,exerting neuroprotective effects.Among them,BYHWD exhibits the most significant therapeutic effect.
BYHWDastragaloside IVischemic strokeratcomparative study
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