补阳还五汤与黄芪甲苷治疗缺血性脑卒中大鼠的疗效比较研究
A Comparative Study on Therapeutic Effects of Buyang Huanwu Decoction and Astragaloside IV in Treatment of Ischemic Stroke in Rats
申杨 1张明蕊 1吴沛泽 1刘静远 1周晓红 2高维娟2
作者信息
- 1. 河北中医药大学,石家庄 050200
- 2. 河北中医药大学,石家庄 050200;河北省心脑血管病中医药防治研究重点实验室,石家庄 050091
- 折叠
摘要
目的:比较补阳还五汤与黄芪甲苷治疗大鼠缺血性脑卒中的疗效.方法:将 50 只SD大鼠随机分组,分为假手术组(Sham组)、模型组(MCAO组)、补阳还五汤组(BYHWD 组)、黄芪甲苷组(AS-IV组)与血塞通组(XueSaiTong组),每组各 10 只.建立大鼠大脑中动脉闭塞(middle cerebral arterial occlusion,MCAO)模型,利用Zea Longa法评估神经功能缺损程度,TTC染色检测脑梗死体积,HE染色观察神经细胞病理形态学改变,尼氏染色观察尼氏体变化,免疫组化染色、免疫荧光染色观察和比较神经元特异性核蛋白NeuN表达.结果:与MCAO组相比,BYHWD组、AS-IV组和XueSaiTong组大鼠神经功能缺损症状均减轻(P＜0.05),脑梗死体积缩小(P＜0.05),神经细胞形态规则,尼氏体数量增多(P＜0.05),NeuN 蛋白表达升高(P＜0.05);与 AS-IV 组相比,BYHWD组神经功能缺损症状有所减轻,但差异无统计学意义(P＞0.05),其余检测结果以BYHWD组疗效较好(P＜0.05).结论:补阳还五汤、黄芪甲苷二者均可起到减轻MCAO大鼠神经功能缺损的效果,发挥神经保护作用;其中补阳还五汤治疗效果更好.
Abstract
Objective:To compare the therapeutic effects of Buyang Huanwu Decoction and Astragaloside IV in the treatment of ischemic stroke in rats.Methods:Fifty SD rats were randomly divided into five groups:sham operation group(Sham group),middle cerebral artery occlusion model group(MCAO group),Buyang Huanwu Decoction group(BYHWD group),Astragaloside IV group(AS-IV group),and Xue Sai Tong group,with 10 rats in each group.The middle cerebral artery occlusion(MCAO)model was es-tablished in rats.The Zea Longa method was used to assess the degree of neurological cleficit,TTC staining to detect the volume of cer-ebral infarction;HE staining to observe pathological morphological changes in nerve cells;Nissl staining to observe changes in Nissl bodies;Immunohistochemical staining and immunofluorescence staining to observe and compare the expression of neuron-specific nucle-ar protein NeuN.Results:Compared with the MCAO group,both the BYHWD group and the AS-IV group showed improvements in neurological deficit symptoms(P＜0.05),reduced cerebral infarction volume(P＜0.05),regular neuronal cell morphology,increased Nissl body counts(P＜0.05),andelevated NeuNproteinexpression(P＜0.05).When compared with the AS-IV group,the BYHWD group displayed a slight reduction in neurological deficit symptoms,but the difference was not statistically significant(P＞0.05).However,the BYHWD group showed more significant therapeutic effects in other detected parameters(P＜0.05).Conclusion:Both BYHWD and Astragaloside IV can alleviate the neurological deficit in MCAO rats,exerting neuroprotective effects.Among them,BYHWD exhibits the most significant therapeutic effect.
关键词
补阳还五汤/黄芪甲苷/缺血性脑卒中/大鼠/比较研究Key words
BYHWD/astragaloside IV/ischemic stroke/rat/comparative study引用本文复制引用
基金项目
国家级大学生创新创业训练计划(202014432001)
河北中医药大学校级燕赵医学项目(YZZZ2023009)
出版年
2024
国家科技期刊平台
NSTL
万方数据