清瘟败毒饮通过TLR4/MyD88/NF-κB信号通路抑制内毒素引起的小鼠急性肺损伤
Qingwen Baidu Yin Inhibiting Endotoxin-induced Acute Lung Injury in mice via TLR4/MyD88/NF-κB Signaling Pathway
武士杰 1马策 1徐智广 2吉立宾 2彭希凤 1程佳慧 1赵志月1
作者信息
- 1. 沧州市人民医院,河北 沧州 061000
- 2. 沧州市医学高等专科学校,河北 沧州 061000
- 折叠
摘要
目的:研究清瘟败毒饮对内毒素引起的急性肺损伤(acute lung injury,ALI)小鼠的保护作用.方法:72只昆明小鼠,随机分为正常组、模型组、地塞米松组、清瘟败毒饮高、中、低剂量组.膜腔注射脂多糖(LPS)建立ALI模型.给药第 3d、第 7d,每组各取 6 只小鼠处死.采集小鼠的血液,分离小鼠肺组织,测定小鼠肺组织湿干比(湿重/干重,W/D);ELISA与PCR检测小鼠血清及肺组织的相关因子表达;观察小鼠肺组织病理变化.结果:与模型组相比,清瘟败毒饮各剂量组小鼠肺组织病理损伤减轻,肺组织W/D下降,小鼠血清的白介素 1(IL-1)、肿瘤坏死因子α(TNF-α),肺组织 Toll 样受体 4(TLR4)、髓样分化因子 88(MyD88)、核因子 κB(NF-κB)、TLR4 mRNA、MyD88 mRNA的表达水平降低,且不同剂量组之间呈剂量依赖性(P＜0.05).结论:清瘟败毒饮对LPS造成的小鼠急性肺损伤有保护作用.
Abstract
Objective:To investigate the protective effects of Qingwen Baidu Yin(QBY)on acute lung injury(ALI)induced by endotoxin in mice.Methods:A total of 72 kunming mice were randomly divided into normal group,model group,dexamethasone group,and high,medium,and low dose groups of QBY.An ALI model was established by intraperitoneal injection of lipopolysaccha-ride.On the 3rd and 7th days of treatment,6 mice from each group were sacrificed.Blood samples were collected,and lung tissues were isolated from the mice.The wet-to-dry ratio of lung tissues was measured.ELISA and PCR were used to detect the expression of relevant factors in serum and lung tissues;and pathological changes in the lung tissues of the mice were observed.Results:Compared with the model group,the pathological damage of lung tissues in the various dose groups of QBY was reduced,with a decrease in the wet-to-dry ratio(W/D)of lung tissues;The expression levels of interleukin-1(IL-1),tumor necrosis factor-alpha(TNF-α)in ser-um,and Toll-like receptor 4(TLR4),myeloid differentiation factor 88(MyD88),nuclear factor-kappa B(NF-κB),TLR4mRNA,and MyD88mRNA in lung tissues were decreased in the various dose groups,showing a dose-dependent effect among different dose groups(P＜0.05).Conclusion:QBY has a protective effect on acute lung injury induced by LPS in mice.
关键词
清瘟败毒饮/急性肺损伤/TLR4/MyD88/NF-κB信号通路Key words
QBY/ALI/TLR4/MyD88/NF-κB signaling pathway引用本文复制引用
基金项目
河北省中医药管理局科研计划项目(2021289)
出版年
2024
国家科技期刊平台
NSTL
万方数据