Objective:To observe the effect of Bushen Tiaojing(kidney-nourishing and menstruation-regulating)prescription on thyroid function and ovarian reserve in mice with controlled ovarian hyperstimulation(COH).Methods:Female ICR mice aged 8-10 weeks were randomly divided into a normal group,a COH group,and a kidney-tonifying group,with 20 mice in each group.Both the COH group and the kidney-tonifying group underwent a COH protocol consisting of gonadotropin-releasing hormone agonist(GnRH-a),human menopausal gonadotropin(HMG),and human chorionic gonadotropin(hCG).In addition,the kidney-tonifying group was ad-ministered the Bushen Tiaojing prescription through gastric gavage concurrently with GnRH-a administration.Radioimmunoassay(RIA)was used to measure serum levels of thyroxine(T4),thyroid-stimulating hormone(TSH),estradiol(E2),and progesterone(P);ELISA was used to detect serum levels of anti-Müllerian hormone(AMH);HE staining was performed to count follicles at different sta-ges in ovarian tissue sections.Results:Compared with the normal group,the COH group mice showed decreased serum levels of T4,AMH,E2,and P(P＜0.05),increased TSH levels(P＜0.05),increased number of corpora lutea,and decreased numbers of primary and secondary follicles(P＜0.05);Compared with the COH group,the kidney-tonifying group mice showed increased serum levels of T4 and AMH(P＜0.05),decreased TSH concentrations(P＜0.05),decreased number of corpora lutea,and increased numbers of prima-ry and secondary follicles(P＜0.05).Conclusion:COH may lead to thyroid dysfunction and decreased ovarian reserve in mice,while the Bushen Tiaojing prescription plays a role in improving thyroid function and ovarian reserve in COH mice.
维普
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