Study of Fuzheng Yiliu Prescription in Regulating Tumor Proliferation,Migration,Invasion,and Apoptosis in Rats with Colon Cancer via Wnt/β-catenin Pathway
Objective:To investigate the effectiveness and mechanism of Fuzheng Yiliu Prescription in regulating tumor prolifera-tion,migration,invasion,and apoptosis in rats with colon cancer(CC).Methods:Thirty-six SPF-grade male rats were selected and ran-domly divided into a control group,a model group,and a low-dose treatment group and a high-dose treatment group,with 9 rats in each group.The control group received no intervention.Rats in the model group and the treatment groups underwent the establishment of a CC model.The model group was then gavaged with saline,while the treatment groups were gavaged with low-dose and high-dose Fuzheng Yi-liu Prescription,respectively.Comparisons were made among the groups in terms of tumor changes,pathological changes,tumor invasion and migration-related indicators,and the expression of tumor cell apoptosis-related proteins.Results:The model group exhibited short-ened colon length accompanied by the formation of numerous colon tumors.In the low-dose group,there was an increase in colon length and a reduction in tumors,while in the high-dose group,the tumor burden was further reduced.HE staining revealed obvious tumor pa-thology in the model group,which was mitigated in the low-dose group and further improved in the high-dose group.Compared to the model group,the levels of vascular endothelial growth factor(VEGF),basic fibroblast growth factor(bFGF),hepatoma-derived growth factor(HDGF),monocyte chemotactic protein-1(MCP-1),transforming growth factor-β(TGF-β),tissue inhibitor of metalloproteinase-1(TIMP-1),matrix metalloproteinase 9(MMP9),tumor necrosis factor receptor-associated protein 1(TRAP1)mRNA,glycogen synthase kinase 3β(GSK3β),cyclin D1,C-myc oncogene pro-tein,anti-apoptotic gene protein Bcl-2(Bcl-2),β-catenin,and microvascular density were lower in both the low-dose and high-dose groups,with the high-dose group showing even lower levels than the low-dose group(P＜0.05).In the low-dose group and high-dose group,the E-cadherin and KRUPPEL-like factor 4(KLF4)in rats were higher than those in the model group,and the levels in the high-dose group were higher than those in the low-dose group(P＜0.05).The model group had an increase in both Bcl-2 and Bcl-2-as-sociated X protein(Bax)expression.Compared with the model group,Bcl-2 expressiom decreased,and Bax expression increased in both the low-dose and high-dose groups,and these changes were dose-dependent manner.Conclusion:Fuzheng Yiliu Prescription effectively inhibits tumor proliferation,migration,and invasion in CC rats,enhances the expression of tumor cell apoptosis-related proteins,and pro-motes tumor cell apoptosis.The mechanism of action may involve regulating downstream factors by inhibiting the activation of the Wnt/β-catenin signaling pathway.
维普
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