Control Effects of Bacillus subtilis and Trichoderma harzianum on Tomato Gray Mold
The use of biological agents to control tomato gray mold can reduce the resistance of pathogenic bac-teria,and is safe and healthy for fruits,without polluting the environment.In order to study the control effects of Bacillus subtilis and Trichoderma harzianum on tomato gray mold,the antagonistic effects of B.subtilis and T.harzianum on the pathogenic bacterium Botrytis cinerea were studied under indoor condition.The control effects of different dosages of B.subtilis and T.harzianum on tomato gray mold was studied under field condition.The in-door confrontation test results showed that both B.subtilis and T.harzianum had antagonistic effects on B.cinerea,and both had a direct destructive effect on its mycelium.The antibacterial effects of sterile filtrate from B.subtilis fermentation gradually decreased with the increasing of dilution ratio.Among them,the antibacterial rate of 10 fold dilution solution reached 80.95％,while the antibacterial rate of 50 fold dilution solution was only 28.57％.The results of field control tests showed that both B.subtilis and T.harzianum had control effects on tomato gray mold.Among them,the best control effect was achieved when the dosage of B.subtilis was 1 125g/hm2 and T.harzianum was 18 000 g/hm2.The control effects on leaves were 84.91％and 85.85％,respectively,and on fruits were 90.00％and 96.00％,respectively.Bio-logical agents B.subtilis and T.harzianum can both control tomato gray mold,reduce the resistance of pathogenic bacteria,ensure the safety and health of fruits,and have no pollution to environment.
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