Diversity Analysis of Endophytes in Cinnamomum septentrionale Based on High-throughput Sequencing
In recent years,the problem of salinization and alkalization of urban green spaces has continued to worsen,and camphor trees,the common green tree species in the south,have shown signs of weakened growth and yellowing of leaves.Wuhan Academy of Landscape Sciences has found that Cinnamomum septentrionale,a plant of the Lauraceae family,has many advantages over other plants of this family,such as good growth,salt alkali resistance,and upright tree shape.It is an excellent tree for landscaping and road greening.In order to further study this excellent green tree,explore the types,functions,and community structure of endophytes in its stems and leaves,high-throughput sequencing technology was used to analyze the biodiversity and microbial community identification of 1-year-old potted C.septentrionale planted in Wuhan Academy of Landscape Sci-ences.The results showed that there were 89 434 and 95 218 effective sequences of endophytic bacteria in the stem and leaves,respectively,with an average length of 406 bp.The Chaol index,Simpson index,and Shannon index of endophytic bacteria in stems were higher than those in leaves.The dominant communi-ties of endophytes in the stem and leaves are Firmi-cutes,Proteobacteria,Cyanobacteria,Bacteroidetes,and Actinobacteria.There was not much difference in the horizontal community structure of endophytes in the leaves and stem.Although there are significant differences in the colony structure of endophytes a-mong different plants,there is a certain degree of similarity in the number of functional genes of endo-phytes.The expression of functional genes in endophytes of C.septentrionale was analyzed,revealing significant differences in the expression of functional genes between the stem and leaves.It is speculated that this is relat-ed to the higher degree of lignification in the stem compared to the leaves,and the significant differences in the colonization environment of endophytes.
万方数据
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