Effect of Electron Beam Irradiation on Degradation Rate of Five Fungal Toxins and Content of Glyc-erol Trioleic Acid Esters in Coix Seed
In order to scientifically utilize electron beam irradiation technology to effectively degrade five fungal toxins in coix seed,including 4 types of aflatoxins:AFB1,AFB2,AFG1,and AFG2,and Zearalenone(ZEA),high-performance liquid chromatography was used to determine the degradation rates of five fungal toxins and the changes in triglyceride content in coix seed after electron beam irradiation at different doses[0(un irradiated),2,4,6,8,and 10 kGy)],as well as the appropriate doses combined with different water content,pH value,and hydrogen peroxide(H2O2).The results showed that the degradation rates of the five toxins in coix seed gradually increased with the increase of irradiation dose.Among them,the degradation rates of the five toxins in 8 and 10 kGy treatments had no significantly difference.Therefore,the appropriate dose for electron beam irradiation to degrade the five toxins in coix seed was determined to be 8 kGy.When irradiated with an electron beam at a dose of 8 kGy,controlling the moisture content of coix seed at 10%to 12%,pH value at 8-9,and adding 1.0%concentration of H2O2,the degradation rate of five fungal toxins in coix seed is high,and the content of glycerol trioleic acid ester can still meet the stan-dards of the Chinese Pharmacopoeia(2020 edition).This research results can provide technical support for the degradation of five toxins in coix seed by electron beam irradiation.