Study on Tissue Culture and Rapid Propagation of Ginger
The key technologies in tissue culture(HgCl2 disinfection time and 6-BA concentration in induced medium)and rapid propagation(hormone concentration in culture medium for simultaneous proliferation and rooting and transplantation substrate components)of the traditional main ginger variety Shizitou in the eastern Hebei region were studied,and the tissue culture rapid propagation technology system was established.The re-sults showed that the suitable duration for soaking and disinfecting explants with HgCl2 was 7 minutes,and the contamination rate of callus tissue was 0.The suitable concentration of 6-BA for inducing culture medium was 2.0 mg/L,with an adventitious bud induction rate of 94% and a contamination rate of 0.The optimal concen-tration of 6-BA and NAA in the culture medium for simultaneous proliferation and rooting was 2.0 mg/L and 0.2 mg/L,respectively,the proliferation rate was 5.14 and the rooting number was 0.99 per plant.The tissue cultured seedlings transplanted on the substrate mixed with vermiculite,peat,and sediment in a volume ratio of 1:1:1 grew strongly with the survival rate of 95%.The key technology for rapid propagation of Shizitou ginger in tissue culture is:soaking and disinfecting ginger sprouts in 0.1%HgCl2 solution for 7 minutes-inoculating callus tissue on MS+6-BA 3.0 mg/L+NAA 0.2 mg/L medium for adventitious bud induction-inoculating adventitious buds on MS+6-BA 2.0 mg/L+NAA 0.2 mg/L medium for simultaneous proliferation and rooting-transplanting tis-sue culture seedlings onto a mixed substrate of vermiculite,peat,and sediment in a volume ratio of 1:1:1 for seedling refinement.
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