Pathogenic Characteristics of Rose Black Spot and Its Sensitivity to Fungicides
Rose is an important ornamental plant,and the occurrence of diseases can seriously affect the ap-pearance quality of rose.Candidate strains were isolated and purified from rose diseased leaf samples using tis-sue separation method.Morphological identification,rDNA-ITS molecular identification,and phylogenetic analy-sis were performed on the strains.In addition,Koch's rule validation and sensitivity analysis of the strains to common fungicides were also conducted.The results showed that the colony and spore morphology of the tested strains were consistent with Alternaria spp.,and rDNA ITS sequence and phylogenetic tree analysis indicated that the tested strains were A.angustifolia.Meanwhile,the Koch's rule experiment showed that the morphologi-cal characteristics of the tested strains and their pathogenicity to rose were completely consistent with those of A.angustifolia.In addition,the analysis of the sensitivity of the tested strains to 10 commonly used fungicides further showed that the tested strains were not sensitive to propiconazole.Fluaziridine,chloramphenicol,prochlo-raz,fluconazole,hexazole,difenoconazole,tebuconazole,fluconazole,and propiconazole had high anti-fungus activity against the tested strains,with an EC50 of 0.059-0.962 μg/mL.The research results provide data support for the identification and chemical prevention and control of rose black spot.
Black spot diseaseRoseMorphologi-cal characteristicsrDNA-ITSSensitivity to fungi-cidesIndoor toxicity
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