Identification of Soft Rot Disease Pathogen of Chinese Cabbage and Inhibition Effect of Bacillus licheniformis XW02
Soft rot is one of the three main diseases of Chinese cabbage,which seriously affects the yield and quality of Chinese cabbage.The use of microorganisms to control soft rot disease of Chinese cabbage has the advantages of being environmentally friendly and not producing drug resistance,which is an inevitable trend for the healthy development of agriculture.Using tissue isolation method,the pathogen were isolated and purified from the soft rot disease strains of Chinese cabbage in the field.The pathogenic strains were screened using pathogenicity test of Chinese cabbage.The 16S rDNA of pathogenic strains were amplified and sequenced,com-paring them with BLAST and GenBank,and a phylogenetic tree was constructed using adjacency method to de-termine their taxonomic status.The results showed that the pathogenic strain of Chinese cabbage soft rot disease was H3,belonging to Pectobacterium carotovorum subsp.carotovorum.The strain XW02 with the best inhibitory effect on H3 was selected from 10 Bacillus licheniformis strains using a double-layer culture method,and the diameter of the inhibition zone reached 49.33 mm.The antibacterial spectrum test and potted plant control effect test of XW02 were conducted.The results of the antibacterial spectrum test showed that XW02 had certain in-hibitory effects on Pseudomonas syringae pv.Lachrymans,Xanthomonas campestris pv.Vesicatoria,Xanthomonas campestris pv.Both campstris and X.citri had cer-tain inhibitory effects.Among them,the antibacterial effect on P.syringae pv.Lachrymans was the great-est,and the antibacterial effect on X.campestris pv.Vesicatoria was the smallest.The results of the potted plant control test showed that XW02 had a good control effect on Chinese cabbage soft rot disease,with a control effect of 60.20％,which was 6.12 percentage points higher than the control agent kasugamycin.Therefore,B.licheniformis XW02 has great biocontrol potential and provides a new resource for the biological control of soft rot disease in Chinese cabbage.
万方数据
维普
