Application of DNA Microarray Chip Method in the Identification of Non-Tuberculous Mycobacterium Species
Objective To investigate the isolation of non-tuberculous mycobacterium(NTM)in Henan Provincial Chest Hos-pital,and to provide a scientific basis for the infection and prevention of NTM in clinical work in this region.Methods 1089 du-plicate specimens(including sputum,lavage solution and sterile bodily fluids of non-respiratory specimens)of suspected my-cobacterium tuberculosis infection in Henan Chest Hospital were detected by DNA microarray chip method,and the distribu-tion characteristics of Mycobacterium species were analyzed.Results In 1089 specimens,878(80.62%)of Mycobacterium tubercu-losis were detected,and 211(19.38%)were detected in NTM.Among them,there were 5 species of non-tuberculous Mycobacteri-um species,153 cases(72.51%)were detected in Intracellular Mycobacteria,28 cases(13.27%)of Mycobacterium avium or-nithus,19 cases(9%)of Mycobacterium purpureum complex,10 cases(4.74%)of Mycobacterium Kansas,and 1 case(0.48%)of Mycobacterium diureum.Among the 211 specimens,127 males(60.19%),84 female patients(39.81%),and the age distribution was mainly elderly,103 cases over 60 years old(48.82%),including 71 male patients(68.93%)and 32 female patients(31.07%);Conclusions 5 kinds of NTM were detected in patients with suspected Mycobacterium tuberculosis admitted to Henan Chest Hospital,and the top three NTM species were Mycobacterium intracellular,Mycobacterium avium and Mycobac-terium turtle complex,and the infected population was mainly elderly men.
万方数据
维普
