Study on the Expression Level of ki-67 in Non-small Cell Lung Cancer,EGFR Mutation and Clinical Correlation
Objective To investigate the expression level of cell proliferation-associated antigen(ki-67)in non-small cell lung cancer(NSCLC)and the mutation of vascular endothelial growth factor receptor(EGFR)and its clinical correlation.Meth-ods 56 patients with NSCLC were collected and divided into groups according to whether ki-67 was positive or not.Tissue samples were collected after surgical resection or biopsy.Immunohistochemistry and Super-ARMS were used to determine the expression of ki-67 and EGFR mutation,respectively.Spearman analyzed the correlation between ki-67 expression level and EGFR mutation and clinic.Results Except gender,age,histological type,lymph node metastasis and distant metastasis,the proportion of moderate/low differentiation and TNM stage Ⅲ+Ⅳ in ki-67 positive group was higher than that in ki-67 nega-tive group,and the difference was statistically significant(P＜0.05).The ratio of wild type in ki-67 positive group was higher than that in ki-67 negative group,and the difference was statistically significant(P＜0.05).Spearman correlation showed that the expression level of ki-67 was negatively correlated with EGFR mutation(r=-0.382,P=0.004),but positively correlated with differentiation degree and TNM stage(r=0.356,P=0.007;r=0.306,P=0.022).Conclusion ki-67 expression and EGFR mutation can both participate in the occurrence and progress of NSCLC,and there is a correlation between ki-67 expression and EGFR mutation,which suggests that they can play a synergistic role.Therefore,the combination of clinical features,ki-67 ex-pression and EGFR mutation can provide key guidance for clinical diagnosis and treatment.
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