摘要
目的 观察二氢辣椒碱对肝细胞癌细胞HepG2的抑制作用,并探讨其分子机制.方法 分为空白组和二氢辣椒碱(100μmol/1、150μmol/L、200μmol/L、250μmol/L)组,应用细胞计数法(CCK-8)检测在不同时间点(24h、48h、72h)、不同浓度二氢辣椒碱(100μmol/L、150μmol/L、200μmol/L、250μmol/L)作用下肝细胞癌细胞HepG2 的活性及数量变化;应用荧光定量PCR法(RT-PCR)检测在不同浓度二氢辣椒碱(150μmol/L、200μmol/L、250μmol/L)作用于肝细胞癌细胞HepG2 24h后,细胞内apoM mRCR的表达水平;应用免疫吸附测定法(ELISA)观察在不同浓度二氢辣椒碱(150μmol/L、200μmol/L、250μmol/L)作用于肝细胞癌细胞HepG2 24h后,细胞上清内的apoM水平.结果 与空白组相比,二氢辣椒碱的浓度在 100μmol/L时,其在各时间点(24、48、72h)的差异都无统计学意义,其余浓度二氢辣椒碱(150μmol/L、200μmol/L、250μmol/L)在各时间点(24、48、72h)差异均有统计学意义,提示肝细胞癌细胞HepG2 对于二氢辣椒碱具有一定的时间及浓度依赖效应;与空白组相比,在浓度为 150μmol/L、200μmol/L、250μmol/L的二氢辣椒碱作用下,肝细胞癌细胞HepG2内的apoM mRNA表达水平明显下调,且肝细胞癌细胞HepG2上清内的apoM水平也有下降(P 均<0.05).结论 二氢辣椒碱对肝细胞癌细胞HepG2 具有抑制作用,且其抑制作用可能与抑制肝细胞癌细胞HepG2 内的apoM表达有关.
Abstract
Objective Observe the inhibitory effect of dihydrocapsaicin on hepatocellular carcinoma cell(HCC)HepG2 cells and explore the molecular mechanism.Method Divided into blank group and dihydrocapsaidine(100μmol/L,150μmol/L,200μmol/L,250μmol/L)group,Cell counting(CCK-8)was used to detect the activity and quantity of HepG2 cells at different time(24h,48h,72h)and different concentrations of dihydrocapsaicin(100μmol/L,150μmol/L,200μmol/L,250μmol/L);Using fluorescence quantitative PCR(RT-PCR)of different concentrations of dihydrocapsaicine(150μmol/L,200μmol/L,250μmol/L)on HepG2 cells for 24h,The expression level of intracellular apoM mRCR;After immunosorbent assay(ELISA),different concentrations(150μmol/L,200μmol/L,250μmol/L)on HepG2 cells,The apoM levels within the cell supernatant.Results Compared to the blank group,Concentration of dihydrocapsaicin at 100μmol/L,No statistically significant differences at each time(24h,48h and 72h),The remaining concentrations of dihydrocapsaicin(150μmol/L,200μmol/L,250μmol/L)were statistically significant at each time(24h,48h,72h),It suggests that HepG2 cells have certain time-and concentration-dependent effects on dihydrocapsaicin;Compared to the blank group,In the presence of dihydrocapsaicin at concentrations of 150μmol/L,200μmol/L,and 250μmol/L,The marked downregulation of apoM mRNA expression levels within HepG2 cells in HCC,Moreover,the apoM level in the supernatant of HCC HepG2 cells also decreased(P<0.05).Conclusion Dihydrocapsaicin has an inhibitory effect on HCC HepG2 cells,and its inhibitory effect may be related to the inhibition of apoM expression in HCC HepG2 cells.
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