The Effect of Dihydrocyline on Hepatocellular Carcinoma Cell HEPG2 APOM Expression
Objective Observe the inhibitory effect of dihydrocapsaicin on hepatocellular carcinoma cell(HCC)HepG2 cells and explore the molecular mechanism.Method Divided into blank group and dihydrocapsaidine(100μmol/L,150μmol/L,200μmol/L,250μmol/L)group,Cell counting(CCK-8)was used to detect the activity and quantity of HepG2 cells at different time(24h,48h,72h)and different concentrations of dihydrocapsaicin(100μmol/L,150μmol/L,200μmol/L,250μmol/L);Using fluorescence quantitative PCR(RT-PCR)of different concentrations of dihydrocapsaicine(150μmol/L,200μmol/L,250μmol/L)on HepG2 cells for 24h,The expression level of intracellular apoM mRCR;After immunosorbent assay(ELISA),different concentrations(150μmol/L,200μmol/L,250μmol/L)on HepG2 cells,The apoM levels within the cell supernatant.Results Compared to the blank group,Concentration of dihydrocapsaicin at 100μmol/L,No statistically significant differences at each time(24h,48h and 72h),The remaining concentrations of dihydrocapsaicin(150μmol/L,200μmol/L,250μmol/L)were statistically significant at each time(24h,48h,72h),It suggests that HepG2 cells have certain time-and concentration-dependent effects on dihydrocapsaicin;Compared to the blank group,In the presence of dihydrocapsaicin at concentrations of 150μmol/L,200μmol/L,and 250μmol/L,The marked downregulation of apoM mRNA expression levels within HepG2 cells in HCC,Moreover,the apoM level in the supernatant of HCC HepG2 cells also decreased(P<0.05).Conclusion Dihydrocapsaicin has an inhibitory effect on HCC HepG2 cells,and its inhibitory effect may be related to the inhibition of apoM expression in HCC HepG2 cells.
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