Regulation of Xin Wumei Pill on Th17/Treg Immune Homeostasis in Mice with Ulcerative Colitis
Objective:To investigate the regulatory effects of Xin Wumei Pill on the immune balance of T helper cell 17(Th17)/Regula-tory T cell(Treg)in mice with ulcerative colitis(UC).Methods:SPF-grade female mice were randomly divided into blank group,model group,Xin Wumei Pill group and Mesalazine group,with 6 mice in each group.Except for the blank group,all mice received 2.5％dextran sulfate sodium salt(DSS)solution freely for 7 d.After the UC model was successfully replicated,the Xin Wumei Pill group(8.085 g·kg-1)and Mesalazine group(606 mg·kg-1)were gavaged with the corresponding drugs,and the mice in the model group were gavaged with PBS for 7 d.HE staining was performed to observe the regulatory effects of colorectal cancer in the mod-el group and the model group.The pathological morphology of the colon was observed and scored by HE staining,the number of Th17(CD4+IL-17+)and Treg(CD4+FOXP3+)cells in mesenteric lymph nodes was detected by flow cytometry,and the number of glu-cose transporter 1(Glut1),hexokinase 2(HK2),and hexokinase 1(HK2)cells in the colon was detected by RT-qPCR.The expres-sion levels of glucose transporter 1(Glut1),hexokinase 2(HK2)and pyruvate kinase isozyme type M2(PKM2)were measured by RT-qPCR.Results:Compared with the blank group,the growth rate of body mass and length of colon decreased and the spleen coeffi-cient increased in the model group(P＜0.01).Compared with the model group,the growth rate of body mass,colon length and spleen coefficient of mice in the Xin Wumei Pill and Mesalazine groups increased(P＜0.01).HE staining results showed that the colonic mu-cosa of mice in the model group was obviously edematous,with the intestinal epithelium peeling off,the villi disappearing,and accompa-nied by the infiltration of inflammatory cells.The glandular structure of the mice in the Xin Wumei Pill and Mesalazine groups was re-stored,and the edema and inflammatory cell infiltration were reduced.Epithelial damage,inflammatory cell infiltration and total score in-creased in mice in the model group compared with the blank group(P＜0.01).Epithelial injury and total score were decreased in mice in the Xin Wumei Pill group compared with that of the model group(P＜0.05).Flow cytometry showed that the number of Th17 in mesenteric lymph nodes increased,the number of Treg decreased,and the ratio of Th17/Treg was elevated in mice in the model group compared with the blank group(P＜0.01).Compared with that of the model group,the number of Th17 in mesenteric lymph nodes de-creased,the number of Treg increased,and the ratio of Th17/Treg decreased in mice in Xin Wumei Pill group and Mesalazine group(P＜0.01).RT-qPCR results showed that the levels of colonic Glut1 mRNA,HK2 mRNA,and PKM2 mRNA in the mice in the model group were elevated when compared with that of the blank group and were treated by neo-Umei pill decreased(P＜0.05).Conclu-sion:Xin Wumei Pill can promote the repair of colonic mucosa in UC mice,and its mechanism may be related to the inhibition of glycol-ysis and the regulation of Th17/Treg immune balance.
万方数据
维普
