Objective:To prepare paeoniflorin-ferulic acid co-loaded transporter,to establish an analytical method for the determina-tion of paeoniflorin-ferulic acid content,to optimize the preparation process of the co-loaded transporter,and to evaluate its pharma-cological properties.Methods:The paeoniflorin-ferulic acid co-loaded transporter was prepared by film-ultrasonic dispersion meth-od.A high performance liquid chromatography(HPLC)method was established for the determination of the content of paeoniflorin and ferulic acid,and the optimal prescription was screened by one-factor investigation and Box-Behnken Design(BBD)-effect surface method using particle size and encapsulation rate of the drug as the indexes,and the morphology,particle size,zeta potential,encapsula-tion rate,drug loading capacity,and storage stability of paeoniflorin-ferulic acid co-loading delivery vehicle were analyzed.Results:The paeoniflorin-ferulic acid co-loaded delivery vehicle prepared with the optimal prescription was light yellow in natural light and had good fluidity.The average particle size of the paeoniflorin-ferulic acid co-loaded deliverer was(287.1±14.4)nm,the zeta po-tential was(-44.3±0.9)mV,the average encapsulation rate of paeoniflorin and ferulic acid were(57.77±3.82)％and(69.41±4.06)％,respectively,and the average drug loading were(5.69±0.46)％and(0.44±0.03)％,respectively.Conclusion:In this ex-periment,a stable paeoniflorin-ferulic acid co-carrier deliverer with certain slow-release performance was prepared,an analytical method for the determination of the content of paeoniflorin and ferulic acid was established,and the preparation process of the paeoni-florin-ferulic acid co-carrier deliverer was preferred,which lays the foundation for the further development of the subsequent paeoni-florin-ferulic acid formulations.
维普
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