Using pyridine as sole carbon source, a high-efficient pyridine-degrading strain B 1 was separated from activated sludge of coking wastewater, and was identified.The optimum degradation conditions were investigated by signal factor experiments.The reaction process was described by kinetics equations.And the degradation effect of pyridine in coking wastewater by strain B1 was examined.The experimental results show that: The strain B1 is gramnegative bacterium identified as gram-negative Acinetobacter sp.;The optimum degradation temperature, initial pH and stirring speed is 30 ℃, 7 and 150 r/min respectively;The pyridine degradation process follows the zero-order kinetics model, and when the initial pyridine mass concentration is 300 mg/L, the degradation rate constant is up to 21.103 mg/(L · h);After the actual coking wastewater with 430 mg/L of initial pyridine mass concentration has been treated for 74 h, the pyridine degradation rate can reach 74.26％.
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