The breakthrough innovation of enterprises is of great significance in enhancing their competitiveness and promoting high-quality economic development.Based on China's venture capital investment data and listed company data from 2010 to 2020,this study empirically investi-gates the impact of venture capital investment on breakthrough innovation of Chinese enterprises.The research finds that venture capital investment has a significant role in enhancing the breakthrough inno-vation of enterprises.Venture capital investment mainly promotes breakthrough innovation by allevi-ating financing constraints,enhancing management experience and improving risk-taking ability.Het-erogeneity tests reveal that the stimulating effect of venture capital investment on breakthrough innova-tion is more pronounced for non-state-owned enterprises,enterprises in the eastern region,and enterprises in high-tech industries.The findings of this paper provide a reference for further improving the regulation policies of venture capital investment,enhancing the development level of venture capital investment and increasing the policy support for breakthrough innovation of enterprises.