基于HPLC-MS/MS的水体与土壤中多类抗生素分析方法研究
Analysis of Multiple Antibiotics in Water and Soil Based on HPLC-MS/MS
胡凌霄 1应红梅 1林欢 1冯加永 1吴佳羽1
作者信息
- 1. 浙江省宁波生态环境监测中心 浙江 315000
- 折叠
摘要
环境中抗生素残留的准确检测对于评估其生态风险具有重要意义.研究建立了基于HPLC-MS/MS(高效液相色谱-串联质谱)技术的水体与土壤中多类抗生素同步分析方法.通过优化色谱分离条件和质谱检测参数,结合固相萃取前处理技术,实现了四环素类、大环内酯类和β-内酰胺类抗生素的高效分离与超痕量检测.方法检出限达到pg/L级水平,线性范围为 0.1～500μg/L,加标回收率为75.8%～95.7%.
Abstract
The accurate detection of antibiotic residues in the environment is important for the assessment of their ecological risk.A syn-chronous analysis of multiple classes of antibiotics in water and soil based on HPLC-MS/MS technology.Efficient separation and supertrace detec-tion of tetracyclines,macrolides and β-lactam antibiotics was achieved by optimizing chromatographic separation conditions and MS detection pa-rameters combined with solid phase extraction pretreatment.Method detection limit reached pg/L level,linear range was 0.1～500 μg/L,and spike recovery rate was 75.8%～95.7%.
关键词
HPLC-MS/MS/水体/土壤/多类抗生素Key words
HPLC-MS/MS/water body/soil/many classes of antibiotics引用本文复制引用
出版年
2025
NETL
NSTL
万方数据