Selection and Identification of Sludge Reduction Enzyme-producing Microorganisms Under Mesophilic and Neutral pH Conditions
The in-situ sludge reduction technology using enzyme-producing microorganisms has gradually gained widespread research attention due to its economic,energy-saving,and eco-friendly advantages.Currently,most experimentally constructed enzyme-producing strains are primarily applicable to extreme environmental conditions such as high temperatures(50~80℃)or alkaline(pH=9~11).These strains have certain limitations when applied to in situ activated sludge reduction in wastewater treatment bioreactors.Based on this,three enzyme-producing bacterial strains with sludge reduction capabilities were identified.Two of them are amylase-producing strains,namely Bacillus thuringiensis DF1(DF1)and Klebsiella huaxiensis DF10(DF10),and one is a protease-producing strain,Chryseobacterium ureilyticum DB8(DB8).The sludge reduction rates achieved by the three strains are as follows:11.35%for DF1,9.72%for DF10,and 11.35%for DB8.