Isolation and Identification of Strawberry Gray Mold Pathogen and Screening of Antagonistic Microorganisms
Strawberry gray mold is one of the important diseases in strawberry production.By i-solating and purifying the pathogen of strawberry gray mold from strawberry bases in Donghai County,Lianyungang and Jiawang District,Xuzhou,a growth dominant pathogen A03 was ob-tained.After the morphological observation and molecular biological identification,it was identi-fied as Botryotinia fuckeliana.A strain of ZXY-1 which had antagonistic effect on A03 was screened by plate confrontative growth method and identified as marine Bacillus amyloliquefa-ciens by molecular biology.ZXY-1 had inhibition radius of 12.67 mm on A03,and inhibition rate of ZXY-1 fermentation liquid on spore germination of A03 was 58.92%.The experiment showed that ZXY-1 fermentation liquid had obvious fresh-keeping effect on strawberry fruit.Therefore,marine Bacillus amyloliquefaciens ZXY-1 has the potential to be used in strawberry production.