Isolation,Identification and Biological Characteristics of Fusarium fujikuroi Species Complex in Maize
In order to clarify the composition characteristics of the pathogen of maize ear rot in China,the samples of maize ear rot were collected continuously in the main maize producing areas of China from 2019 to 2021,and the pathogen was isolated by conventional tissue isolation and culture method.The screened strains were identified based on morphology and molecular biolo-gy,and their biological characteristics were analyzed.A total of 618 strains of Fusarium fujiku-roi species complex(FFSC)belonging to 6 species were obtained,of which F.verticillioides was the dominant species.The separation frequency was 85.11％.The separation frequencies of the other five species were F.fujikuroi 2.27％,F.proliferatum 10.03％,F.andiyazi 0.65％,F.temperatum 1.29％,F.subglutinans 0.65％.Biological characterization showed that F.verticil-lioides had the fastest mycelial growth rate and the strongest asexual reproduction ability.The 58 strains could synthesize fusaric acid(FA)in vitro,and the content of FA in F.andiyazi was the highest.F.fujikuroi,F.proliferatum and F.verticillioides can synthesize high concentrations of fumonisin B(FB)in vitro culture;There was no significant difference in the content of bikaverin(BIK)among dif-ferent species(P＞0.05).F.fujikuroi,F.proliferatum and F.temperatum mainly produced beau-verin(BEA);The ability of F.andiyazi and F.fujikuroi to synthesize MON was significantly higher than that of F.subglutinans and F.proliferatum(P＜0.05).The infection of maize ears showed that the pathogenicity of F.subglutinans was significantly higher than that of other species(P＜0.05).This study clarified the main pathogen types of maize ear rot in China and the pathogenicity and toxin-producing ability of its representative strains.
maize ear rotFusarium fujikuroi species complexisolation and identificationbio-logical characteristics
万方数据
维普
