鳗弧菌(Vibrio anguillarum)是水产养殖中常见病原菌之一.为探究鳗弧菌感染海洋经济动物单环刺螠(Urechis unicinctus)后宿主基因表达水平的变化和应答细菌感染的分子机制,运用高通量测序技术对感染鳗弧菌的单环刺螠对照组、易感组和抗感组体腔细胞进行了转录组测序,并对比分析其差异表达基因(DEGs)及信号通路.结果显示,鳗弧菌感染单环刺螠后,易感组体腔细胞差异表达基因数目为2 309个,其中1 451个基因表达上调,858个基因表达下调;抗感组中DEGs数目为1 955个,其中1 311个基因表达上调,644个基因表达下调.KEGG通路富集分析显示,与免疫相关的通路主要有MAPK信号通路、过氧化物酶体、内吞作用、溶酶体、氨基酸生物合成等,筛选出的潜在抗感基因主要有牛磺酸分解代谢双加氧酶(Taurine catabolism dioxygenase,TauD)、细胞色素P450(Cyto-chrome P450,CYP450)、Casitas B 细胞淋巴瘤 B(Casitas B lymphoma-b,Cbl-b)、肌动蛋白相关蛋白 3(Actin-related protein 3,ACTR3)、热休克蛋白 70(Heat shock protein 70,HSP70)、氨基酸通透酶(A-mino acid permease,AAPs)、编码葡萄糖激酶(Glucokinase,GCK)、乙醛酸和羟基丙酮酸还原酶(Glyoxylate reductase/hydroxy pyruvate reductase,GRHPR)、胱硫醚 β-合酶(Cystathionine beta-syn-thase,CBS)、组氨酸磷酸酶(Histidine phosphatase,LHPP)、谷草酰乙酸转氨酶 2(Glutamic-oxaloacetic transaminase 2,GOT2)、乳酸脱氢酶(Lactate dehydrogenase,LDH)和细胞分裂周期 42(Cell division control protein 42,CDC42),易感基因主要有 ADP 核糖激化因子(ADP-ribosylation factor,ARF)、细胞粘附素(Cytohesin,CYTH)、EH 结构域蛋白 1(EH domain-containing protein 1,EHD1)、Ras 相关蛋白Rab-8A(Ras-related protein Rab-8A,Rab8A)、钙网蛋白(Calreticulin,CALR)和 Ras 相关的 C3 肉毒素底物1(Ras-related C3 botulinum toxin substrate 1,RAC1).实验结果可为鳗弧菌对水产养殖动物的感染机制及单环刺螠对病原菌的免疫应答研究提供数据参考.
Transcriptomic Analysis of the Coelomocytes of Urechis unicinctus Infected with Vibrio anguillarum
Vibrio anguillarum is one of the common pathogenic bacteria in aquaculture.In order to investigate the changes of host gene expression level and the molecular mechanism of response to bacterial infection after V.anguillarum infected marine economic animal Urechis unicinctus,the high-throughput sequencing technology was adopted to sequence the transcriptomes of coelo-mocytes of different groups of U.unicinctus after infected with V.anguillarum,including con-trol group,susceptibility group and disease-resistance group,and then the differently expressed genes(DEGs)and signal channels were comparative analyzed in this study.The results showed that the number of DEGs in the susceptible group was 2 309,including 1 451 up-regulated genes and 858 down-regulated genes,and the number of DEGs in the disease-resistance group was 1 955,including 1 311 up-regulated genes and 644 down-regulated genes,compared with the con-trol group.The KEGG pathway enrichment analysis showed that the immune-related pathways mainly included MAPK signaling pathway,peroxisome,endocytosis,lysosome and amino acid biosynthesis,and the potential resistance genes mainly included Taurine catabolism dioxygenase(TauD),Cytochrome P450(CYP450),Casitas B lymphoma-b(Cbl-b),Actin-related protein 3(ACTR3),Heatshock protein 70(HSP70),Amino acid permease(AAPs),Glucokinase(GCK),Glyoxylate reductase/hydroxypyruvate reductase(GRHPR),Cystathionine beta-syn-thase(CBS),Histidine phosphatase(LHPP),Glutamic-oxaloacetic transaminase 2(GOT2),Lactate dehydrogenase(LDH),Cell division control protein 42(CDC42),while the susceptibili-ty genes mainly included ADP-ribosylation factor(ARF),Cytohesin(CYTH),EH domain-con-taining protein 1(EHD1),Ras-related protein Rab-8A(Rab8A),Calreticulin(CALR)and Ras-related C3 botulinum toxin substrate 1(RAC1).This study may provide data referenced for fur-ther exploring the mechanism of infection against aquaculture animals by V.anguillarum and the immune response of U.unicinctus to pathogenic bacteria.
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