Optimization of the Fermentation Conditions for Cottonseed Meal by Fish-derived Bacillus subtilis J5
In order to investigate the effect of fish-derived Bacillus subtilis J5 on the elimination of anti-nutritional factors and the nutritional composition of cottonseed meal under different fer-mentation conditions,Bacillus subtilis J5 from the gut of common carp was used as a fermenta-tion strain in the experiment.An orthogonal experiment L9(34),four-factors and three-levels was designed to obtain the optimal combination of temperature,humidity,inoculation amount,and fermentation duration for the best fermentation effect of cottonseed meal.The results showed that the highest detoxification rate of free gossypol in the cottonseed meal was achieved when the humidity was 60%,and the inoculation amount was 5%at 30℃for 4 d fermentation.Under this condition,94.8%free gossypol in the cottonseed meal was eliminated with a signifi-cant decrease of phytic acid content while the crude protein content of cottonseed meal significant-ly increased.The composition and amount of amino acids in the fermented cottonseed meal groups were superior to those in the unfermented group.In conclusion,the optimum fermenta-tion conditions of Bacillus subtilis J5 for cottonseed meal were 60%humidity combined with 5%inoculation amount at 30℃for 4 d fermentation.
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