鱼源枯草芽孢杆菌J5发酵棉粕条件优化
Optimization of the Fermentation Conditions for Cottonseed Meal by Fish-derived Bacillus subtilis J5
徐一力 1戴子凡 1陈铭川 1丁皓天 1吴冰 1纪逸 1陈香凝 1丁祝进 1魏朝青 1程汉良 1孙宜防 2许建和1
作者信息
- 1. 江苏海洋大学 海洋科学与水产学院,江苏 连云港 222005
- 2. 沭阳县周集乡孙宜防水产养殖专业合作社,江苏 宿迁 223699
- 折叠
摘要
为了探究鱼源枯草芽孢杆菌J5 在不同发酵条件下对棉粕中抗营养因子去除效果和棉粕营养组分影响,以鱼源枯草芽孢杆菌J5 为发酵菌种,依据温度、湿度、接种量和发酵时间设计 4 因素 3水平正交试验L9(34).结果表明,温度 30℃,湿度 60%,接种量 5%,发酵 4 d,游离棉酚脱毒率最高达 94.8%,植酸含量显著下降,同时发酵后棉粕中粗蛋白含量显著上升.以游离棉酚、植酸、粗蛋白主效应分析结果得到的 3 组发酵条件发酵后,与未发酵组相比发酵棉粕中氨基酸含量和组成均优于其他组别.因此,鱼源枯草芽孢杆菌J5 对棉粕最适发酵条件为温度 30℃、湿度 60%、接种量 5%、发酵时间 4 d.
Abstract
In order to investigate the effect of fish-derived Bacillus subtilis J5 on the elimination of anti-nutritional factors and the nutritional composition of cottonseed meal under different fer-mentation conditions,Bacillus subtilis J5 from the gut of common carp was used as a fermenta-tion strain in the experiment.An orthogonal experiment L9(34),four-factors and three-levels was designed to obtain the optimal combination of temperature,humidity,inoculation amount,and fermentation duration for the best fermentation effect of cottonseed meal.The results showed that the highest detoxification rate of free gossypol in the cottonseed meal was achieved when the humidity was 60%,and the inoculation amount was 5%at 30℃for 4 d fermentation.Under this condition,94.8%free gossypol in the cottonseed meal was eliminated with a signifi-cant decrease of phytic acid content while the crude protein content of cottonseed meal significant-ly increased.The composition and amount of amino acids in the fermented cottonseed meal groups were superior to those in the unfermented group.In conclusion,the optimum fermenta-tion conditions of Bacillus subtilis J5 for cottonseed meal were 60%humidity combined with 5%inoculation amount at 30℃for 4 d fermentation.
关键词
发酵棉粕/枯草芽孢杆菌J5/正交试验/棉酚Key words
fermented cottonseed meal/Bacillus subtilis J5/orthogonal experiment/gossypol引用本文复制引用
基金项目
江苏省科技支撑项目(苏北科技专项)(SZ-SQ2021034)
江苏省自然科学基金资助项目(BK20201465)
江苏省研究生科研与实践创新计划项目(SJCX22_1663)
出版年
2024
NETL
NSTL
万方数据