Transcriptome Sequencing and Analysis of Hypsizygus marmoreus Fruiting Body
In the study,transcriptome sequencing was performed for the mature fruiting bodies of Hyp-sizygus marmoreus using Illumina technology.A total of 38 112 transcripts were obtained after data assembly and optimization evaluation,and after removing redundancy,16 580 unigenes were obtained.By comparing the unigene sequences with databases such as Nr,GO,and KEGG,a to-tal of 12 655 unigenes obtained functional annotations,accounting for 76.33%.Based on GO and KEGG databases,unigenes that obtained functional annotations were divided into 38 and 22 func-tional subcategories.In the GO molecular functional category,it was found that these unigenes were mainly classified into catalytic activity,binding and transport activity,which was similar to the molecular functional categories of various edible mushrooms such as Lentinula edodes and Auricularia cornea.On this basis,key genes involved in the post harvest physiological processes were excavated and analyzed.A large number of key genes involved in metabolic pathways such as respiration and cell wall degradation were found.166 transcription factors were identified,a-mong which zinc finger proteins were the most abundant type of transcription factors,with a to-tal of 78.The results provide important theoretical support for post harvest molecular regulation of edible mushrooms.
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万方数据
