Role and Mechanism of WXJ-112 Targeting CDK4/6 in Breast Cancer Cells
Triple-negative breast cancer(TNBC)is a heterogeneous recurrent cancer associated with high metastasis,poor prognosis,and a lack of therapeutic targets,while inhibition of CDK4/6 targets produces anti-tumor effects.In the present study,a novel Abemaciclib derivative named WXJ-112 was designed and synthesized.The effect of compound WXJ-112 on several as-pects of TNBC biology was investigated.Cell viability was measured by the methylthiazolium tetrazolium(MTT)assay and clone formation,migration,and invasion ability was detected by clone formation assay,scratch assay,and invasion assay.Western blot analysis was performed to detect the expression of proteins related to the CDK4/6-Rb-E2F pathway.The tumor suppressor effect was investigated in a chicken embryo transplantation tumor model.The experimental re-sults showed that WXJ-112 inhibited the proliferation,colony formation,migration,and invasion of MDA-MB-231 cells,induced cycle blockade,and acted as a significant anti-tumor activity in the chicken embryo transplantation tumor model.In summary,compound WXJ-112 has good an-ti-tumor cell proliferation effects and can be used as a promising drug candidate for breast cancer treatment.
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