Exploring dynamic expression of IL-34 in mouse model of chronic apical periodontitis
Objective:To explore the expression and changes of IL-34 in mouse model of chronic apical periodontitis.Methods:35 SD mice were selected to create a model of apical periodontitis by pulp opening of their mandibular first molar,and they were randomly decapitated and killed on days 0,7,14,21,and 28.The mandible containing the first molar was anatomically separated and X-ray imaging examinations were conductd to observe the periapical damage in the model.The mandibular specimens were prepared for sectio-ning,and HE staining was used to observe the histopathology changes around the root tip,and tartrate resistant acid phosphatase(TRAP)staining was used to detect osteoclasts.The dynamic expression of IL-34 and TNF-α in a periapical periodontitis model were detecetd by real-time fluorescence quantitative polymerase chain reaction(RT-qPCR).Results:X-ray examination indicated that there was no obvious abnormalities in the apical area from 0 to 7d;mild apical damage began to appear from 14d to 21d;on 28d,the root tip shadow became larger and the range widened.HE staining showed that the periapical tissue was normal and no significant changes were observed on 0d;the infiltration of inflammatory factors began to increase on 7d;on 14d,obvious bone destruction and necrotic centers appeared;on 21d,there was a decrease in inflammatory exudation and an increase in small areas of bone destruction;after 28d,the infiltration of inflammatory cells tended to stabilize,with small-scale bone destruction connecting into patches,and large-scale bone de-struction forming.TRAP staining showed that there was almost no osteoclast on 0d,the number of osteoclast increased significantly from 7d to 14d,reached the peak on 21d,and slightly decreased on 28d,but was higher than 0d on 7d,14d,21d,and 28d(P<0.05).RT qPCR detection showed that the mRNA expression levels of IL-34 and TNF-α from 7d to 28d were all gradually increased,reaching their peak on 14d,showing a decreasing trend on 21d,and then showing an upward trend on 28d.And the expression levels of both from 7d to 28d were higher than those of 0d(P<0.05),showing a positive correlation.Conclusion:The mRNA expression level of IL-34 changes with the development of periapical inflammation,especially in relation to the degree of bone destruction and TNF-α.There is a close connection,and a brief decrease in expression indicates that its expression has been suppressed,which provides direc-tion for our clinical treatment.
万方数据
维普
