Research of role of Huangqi injection in treatment of spinal cord injury based on network pharmacology
Objective:To investigate the potential role of Huangqi injection in the treatment of spinal cord injury(SCI),which may provide a reference for the treatment of SCI and new drug development.Methods:The active ingredients of Astragalus were collected from the published literature.Potential target genes of Huangqi and SCI were retrieved from PharmMapper and GeneCards databases,respectively.After crossing the target genes of Astragalus and SCI through Venn diagram,the intersectional target genes were uploaded to STRING database for the protein-protein interaction network analysis,followed by a construction of drug-active component-target-dis-ease network.Gene ontology(GO)functional enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of intersectional target genes were performed using Metascape database.Results:There were 289 Astragalus targets,3332 SCI targets and 157 drug-disease co-targets were obtained for the action of Astragalus on SCI,including albumin(ALB),AKT serine/threonine kinase 1(AKT1),epidermal growth factor receptor(EGFR),non-receptor tyrosine kinase(SRC),and cysteinyl as-partate specific proteinase 3(CASP3).Go analysis mainly involved the response to hormone,positive regulation of cell migration,re-productive structure development,response to bacterium.KEGG pathways mainly involved the pathways in cancer,prostate cancer,lipid and atherosderosis,adherens junction,bladder cancer.Conclusion:Astragalus may suppress the onset and progression of SCI through multiple targets and pathways.
Spinal cord injuryNetwork pharmacologyAstragalusMechanism of action
万方数据
维普
