To investigate the identification of microplastics in wheat flour,the study was based on micro Fourier infrared spectroscopy.Firstly,the detection mode and digestion solution were optimized,and the tolerance of 4 common plastics(polyethylene,polypropylene,nylon 6,and polystyrene)before and after digestion.Additionally,the composition of microplastics in commercially available wheat flour was analyzed,the abundance and contamination pathways of microplastics in the wheat flour samples were analyzed.The results showed that:(1)A total of 288 suspected microplastic substances were identified in the wheat flour samples,with an average length of(912±527)μm.The qualitatively identified compositions were polyester,cellulosic,polypropylene,and nylon 6.The identified substances were dominated by polyester and cellulosic,of which polyester accounted for 21.5%,and cellulosic accounted for 52.4% .(2)Suspected microplastic substances,predominantly transparent and blue color were selected,accounting for 54.2% and 22.6%,respectively.In the transparent materials,polyester comprised 29.0%,while cellulosic made up 43.5%;In the blue materials,polyester accounted for 16.9%,and cellulosic constituted 44.6% .It was suggested that the source of microplastic contamination in wheat flour may be linked to human daily activities and packaging materials.The study represented the first qualitative identification of microplastics in wheat flour,with methods applicable to characterizing the 4 types of microplastics larger than 20 μm,which was helpful to evaluate the health risk of microplastics exposure in wheat flour.