氯及氯胺消毒方式下管壁生物膜生长特性和群落功能研究
GROWTH CHARACTERISTICS AND COMMUNITY FUNCTION OF PIPE WALL BIOFILMS UNDER CHLORINATION/CHLORAMINATION
张心悦 1孔赟 1施卫红 1黄咏洲1
作者信息
- 1. 江苏省规划设计集团市政规划与工程设计院,南京 210019
- 折叠
摘要
基于生物膜环状反应器,模拟培养氯消毒和氯胺消毒体系下的管壁生物膜,从生长特性、群落结构和功能丰度等方面探究管壁生物膜特性,研究不同消毒方式(氯和氯胺)胁迫下生物膜的特性差异.结果表明:与氯相比,氯胺消毒下生物膜的单位面积生物量更高,且活菌占比更大,表明在相同浓度水平下,氯比氯胺更能抑制管壁微生物的生长繁殖和细胞活性.氯胺消毒下生物膜的群落丰富度和多样性高于氯消毒,2种消毒方式下的生物膜群落结构和功能丰度存在显著差异.氯消毒下,γ-变形菌纲(平均相对丰度63.8％)占据主要优势,Nevskia属(32.9％)和嗜甲基菌属(20.4％)为优势菌属;氯胺消毒下,α-变形菌纲(53.8％)为最优势菌纲,慢生根瘤菌属(10.4％)为优势菌属.与氯胺相比,氯消毒下生物膜的新陈代谢功能丰度更具优势,尤其是在氨基酸代谢、碳水化合物代谢和脂类代谢关乎有机物利用的功能方面,分别增加36％、41％和48％,为供水管网中的微生物污染控制和以生物膜为前体物的副产物生成控制提供了一定理论基础.
Abstract
Based on the biofilm annular reactor,this study simulated pipe wall biofilm in the chlorine disinfection and chloramine disinfection system.The impact of disinfection(chlorination/chloramination)on the growth characteristics,community composition,and functional abundance of the microbial was explored.The results showed that in comparison with chlorine,the biofilm biomass in the chloramine disinfection system was higher,and the proportion of live bacteria was larger.The community richness and diversity of biofilm under chloramination were higher than that under chlorination.There were significant differences in the community structure of biofilm under the two disinfection methods.Under chlorination,Gammaproteobacteria(63.8％)were predominant,and Nevskia(32.9％)and Methylophilus(20.4％)were the dominant genera;under chloramination,Alphaproteobacteria(53.8％)was the most dominant class,and Bradyrhizobium(10.4％)was the dominant genus.Compared with chloramination,the metabolism functional abundance of the biofilms in the chlorine disinfection system was more advantageous,especially in amino acid metabolism,carbohydrate metabolism,and xenobiotics metabolism.In terms of amino acid metabolism functional pathway,there were significant differences in the functional abundance of tryptophan metabolism,β-alanine metabolism,lysine degradation,and degradation of valine,leucine,and isoleucine for the biofilms under the two disinfection methods.
关键词
管壁生物膜/氯消毒/氯胺消毒/群落结构/功能丰度Key words
pipe wall biofilm/chlorination/chloramination/community structure/functional abundance引用本文复制引用
基金项目
水体污染控制与治理科技重大专项(2014ZX07405002)
出版年
2024
NETL
NSTL
万方数据