苯诱导石油降解红平红球菌(Rhodococcus erythropolis)KB1形成VBNC状态及复苏特征研究
FORMATION OF VIABLE BUT NON-CULTURABLE STATE OF RHODOCOCCUS ERYTHROPOLIS KB1 INDUCED BY HIGH CONCENTRATION BENZENE AND CHARACTERISTICS ITS RESUSCITATED CELLS
陈诗源 1陈吉祥 2王永刚 3杨言武 1张博文1
作者信息
- 1. 兰州理工大学石油化工学院,兰州 730050
- 2. 兰州理工大学石油化工学院,兰州 730050;兰州理工大学生命科学与工程学院,兰州 730050
- 3. 兰州理工大学生命科学与工程学院,兰州 730050
- 折叠
摘要
为研究高浓度苯诱导石油降解菌红平红球菌KB1形成的活的非可培养状态(VBNC)及VBNC状态细胞复苏后的生理特性和石油降解能力,采用LB培养基和基础培养基培养红平红球菌KB1,通过紫外分光光度法、扫描电镜和透射电镜观测苯诱导下红平红球菌KB1的VBNC状态形成过程及特征,用红外分光光度法测定复苏后细胞石油降解能力.结果表明,高浓度苯对红平红球菌KB1生长有明显抑制作用,在苯浓度2.5％培养液中6 h后红平红球菌KB1进入VBNC状态,细胞形态发生变化,但结构完整.VBNC状态的细胞在一定条件下复苏为可培养形式,复苏细胞保持了良好环境适应能力和较高石油降解能力.
Abstract
To study the inducing effects of different concentrations of benzene on the oil-degrading bacterium R.erythropolis KB1,characterize the benzene-induced VBNC state of the bacterial cells,and physiological and biochemical characteristics of bacterial cells,and petroleum degradation ability of the resuscitated R.erythropolis cells,R.erythropolis KB1 was cultured in LB medium and basal medium.The formation of the VBNC state of R.erythropolis KB1 cells induced by benzene was observed by spectrophotometry,scanning electron microscopy,and transmission electron microscopy,and the oil degradation abilities of the resuscitated bacterial cells were determined by infrared spectrophotometry.The results showed that when the concentration of benzene in the culture medium was 2.5％above,the growth of R.erythropolis KB1 could be effectively inhibited.After being cultured for 6 h,R.erythropolis KB1 cells entered the VBNC state.The VBNC cells could be resuscitated into the culturable state under certain conditions.The resuscitated bacterial cells showed good adaptabilities to the environments,and their oil degradation ability was not changed obviously.
关键词
红平红球菌/苯/活的非可培养状态(VBNC)/复苏Key words
Rhodococcus/benzene/VBNC/resuscitation引用本文复制引用
基金项目
国家自然科学基金(31760028)
国家自然科学基金(42267017)
出版年
2024
NETL
NSTL
万方数据