FORMATION OF VIABLE BUT NON-CULTURABLE STATE OF RHODOCOCCUS ERYTHROPOLIS KB1 INDUCED BY HIGH CONCENTRATION BENZENE AND CHARACTERISTICS ITS RESUSCITATED CELLS
To study the inducing effects of different concentrations of benzene on the oil-degrading bacterium R.erythropolis KB1,characterize the benzene-induced VBNC state of the bacterial cells,and physiological and biochemical characteristics of bacterial cells,and petroleum degradation ability of the resuscitated R.erythropolis cells,R.erythropolis KB1 was cultured in LB medium and basal medium.The formation of the VBNC state of R.erythropolis KB1 cells induced by benzene was observed by spectrophotometry,scanning electron microscopy,and transmission electron microscopy,and the oil degradation abilities of the resuscitated bacterial cells were determined by infrared spectrophotometry.The results showed that when the concentration of benzene in the culture medium was 2.5％above,the growth of R.erythropolis KB1 could be effectively inhibited.After being cultured for 6 h,R.erythropolis KB1 cells entered the VBNC state.The VBNC cells could be resuscitated into the culturable state under certain conditions.The resuscitated bacterial cells showed good adaptabilities to the environments,and their oil degradation ability was not changed obviously.
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