基于甲烷氧化菌的地下水硝酸盐还原效能及功能微生物研究
RESEARCH ON GROUNDWATER NITRATE REDUCTION EFFICIENCY BASED ON METHANOTROPH AND FUNCTIONAL MICROORGANISMS
廖珣 1李彦澄 2张玉多 1杨启林 1李江2
作者信息
- 1. 贵州大学资源与环境工程学院喀斯特地质资源与环境教育部重点实验室,贵阳 550025
- 2. 贵州大学资源与环境工程学院喀斯特地质资源与环境教育部重点实验室,贵阳 550025;贵州喀斯特环境生态系统教育部野外科学观测研究站,贵阳 550025
- 折叠
摘要
地下水中污染物成分复杂,来源广泛,针对地下水中的硝酸盐污染物,利用生物膜反应器,开展了基于甲烷氧化菌的地下水硝酸盐还原效能、功能微生物和代谢途径分析.研究发现,在进水ρ(NO3--N)为10 mg/L时,系统对硝酸盐的最高去除率达到98.83％,实现硝酸盐的有效去除.通过溶解性有机物(dissolved organic matter,DOM)组分变化分析发现出水中DOM主要是类色氨酸蛋白质类物质.宏基因组分析结果表明,生物膜反应器甲烷氧化系统中的好氧甲烷氧化菌主要为Methylocystis(5.25％)、Methylomonas(2.73％),厌氧甲烷氧化菌主要为Methylomirabilis(0.0016％)、Methanoperedens(0.0016％),硝酸盐还原菌主要为 Lysobacter(9.72％)、Opitutus(2.74％)、Hyphomicrobium(2.01％).利用KEGG数据库对系统中微生物的功能进行注释,识别出多种相关功能基因(40种甲烷代谢相关的功能基因和19种氮代谢相关的功能基因)以及较完善的甲烷代谢通路(5条)和氮代谢通路(6条),证明甲烷氧化菌具有部分反硝化的能力,对硝酸盐具有良好的选择性.相关研究结果可为硝酸盐污染地下水修复提供技术支撑和理论依据.
Abstract
Pollutants in groundwater are complicated from many sources,and nitrate pollutants are one of them.In this study,a methanotroph-based biofilm reactor was established to find a remediation method with low consumption,high efficiency,and no secondary pollutants,by assaying nitrate reduction efficiency,functional microorganisms,and metabolic pathway.Results showed that the maximum nitrate removal rate reached 98.83％when nitrate nitrogen concentration in the influent was 10 mg/L.DOM component changes found that the dissolved organic matter primarily was a tryptophan-like protein.Macrogenomic analysis revealed that the dominant aerobic methanotrophs in this biofilm reactor were Methylocystis(5.25％)and Methylomonas(2.73％);the dominant anaerobic methanotrophs were Methylmirabilis(0.0016％),and Methanoperedens(0.0016％);and the dominant nitrate-reducing bacteria were mainly Lysobacter(9.72％),Opitutus(2.74％),and Hypomicrobium(2.01％).The KEGG database was annotated to identify microbial functions in the system,including 40 methane-metabolism-related functional genes and 19 nitrogen-metabolism-related functional genes,as well as 5 relatively complete methane metabolism pathways and 6 nitrogen metabolism pathways.Thus,methanogens could be proved with the capability of partial denitrification and good selectivity for nitrate.Relevant research results can provide technical support and a theoretical basis for the remediation of nitrate-contaminated groundwater.
关键词
甲烷氧化菌/地下水/硝酸盐/宏基因组学/功能微生物Key words
methane oxidizing bacteria/groundwater/nitrate/metagenomics/functional microorganism引用本文复制引用
基金项目
国家自然科学基金青年科学基金项目(52100033)
贵州省科技计划项目(黔科合支撑[2021]一般475)
出版年
2024
NETL
NSTL
万方数据