六价铬胁迫对李氏禾根系分泌物和微生物组成的影响
EFFECT OF Cr(Ⅵ)STRESS ON ROOT EXUDATES AND MICROBIAL COMPOSITION OF LEERSIA HEXANDRA SWARTZ
曹栢枫 1刘子昕 1韦翠屿 1唐雨菲 1石玉翠 2蒋萍萍1
作者信息
- 1. 桂林理工大学地球科学学院,广西桂林 541004
- 2. 桂林理工大学环境科学与工程学院,广西桂林 541004
- 折叠
摘要
为探讨Cr(Ⅵ)与李氏禾根系分泌物组分的相互作用关系,并找出与Cr(Ⅵ)还原相关的关键微生物,研究了 6个Cr(Ⅵ)处理浓度(0,40,60,80,100,120 mg/L)对人工湿地微生物燃料电池耦合系统(CW-MFC)中李氏禾根系分泌的有机物种类及含量的影响,同时测定了对照和最优铬处理浓度下系统中的微生物群落.结果表明:1)铬胁迫对李氏禾根系分泌物的影响呈先增加后降低的趋势,当Cr(Ⅵ)处理浓度为80 mg/L时,根系分泌物的化合物最多.2)根系分泌物以脂类、烷类、酚类、烯类和醇类物质为主,其中烷类物质居多.3)六价铬胁迫显著增加了 Geobacter在CW-MFC系统中的丰度,这可能是李氏禾中参与铬还原和富集相关的关键微生物.
Abstract
To investigate the response mechanism of root secretions of Leersia hexandra Swartz to Cr(Ⅵ)and identify the key microorganisms in Cr(Ⅵ)reduction,in this study,the types and contents of organic matters secreted by the roots of L.hexandra in an artificial wetland-microbial fuel cell(CW-MFC)system were studied at six Cr(Ⅵ)treatment levels(0,40,60,80,100,120 mg/L).Soil microorganisms in the system were measured simultaneously at the control and optimal chromium treatment concentrations.The results showed that:1)the effect of chromium stress on the root secretion of L.hexandra showed an increasing trend followed by a decreasing trend during the increase of chromium concentration,with the highest quantity of compounds in the root secretion at a hexavalent chromium treatment concentration of 80 mg/L;2)the roots of L.hexandra were mainly lipids,alkanes,phenols,alkenes,and alcohols,of which alkanes were the most abundant;3)hexavalent chromium stress significantly increased the enrichment of Geobacter in the CW-MFC system,which may be the key microorganism associated with chromium reduction and enrichment in L.hexandra.
关键词
李氏禾/六价铬胁迫/根系分泌物/土壤微生物Key words
Leersia hexandra Swartz/Cr(Ⅵ)stress/root exudates/soil microorganism引用本文复制引用
基金项目
国家自然科学基金项目(52170154)
国家自然科学基金项目(52230006)
广西自然科学基金项目(2021GXNSFBA196023)
广西自然科学基金项目(2020GXNSFDA297018)
出版年
2024
NETL
NSTL
万方数据