CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS
About one million tons of CS2 is used in the production of viscose fiber in China every year.CS2 is often removed by aeration in industry.However,some CS2 remains in the water and enters the nitrification unit of wastewater treatment,affecting operational performance.It was found that 10 mg/L CS2 significantly inhibited nitrification sludge activity under short-term stress,low concentration of CS2(10 to 40 mg/L)could promote the metabolic activity and antioxidant activity of nitrification sludge,and high concentration of CS2(100 to 200 mg/L)could inhibit the activity.The nitrification reaction was completely inhibited under long-term CS2 stress(20 to 200 mg/L),and white turbidity was found on the surface of nitrification sludge during long-term operation.The white turbidity was analyzed as simple sulfur by liquid chromatography and XPS,and the assimilation process of CS2→COS→H2S→S0→SO32-→SO42-might occur.In 16S rRNA analysis,it was found that the abundance of Actinobacteria and Sinomonas involved in sulfur oxidation has increased,which also verified this hypothesis.
carbon disulfidenitrificationmetabolism activityoxidative stressmicrobial community
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